Issue 39, 2011
From the journal:

Physical Chemistry Chemical Physics

Interaction strength between proteins and polyelectrolyte brushes: a small angle X-ray scattering study†

Katja Henzler,ab   Björn Haupt,ab   Sabine Rosenfeldt,b   Ludger Harnau,cd   Theyencheri Narayanane  and  Matthias Ballauff*af  

* Corresponding authors

a Soft Matter and Functional Materials, Helmholtz-Zentrum Berlin für Materialien und Energie GmbH, Hahn-Meitner-Platz 1, 14109 Berlin, Germany
E-mail: matthias.ballauff@helmholtz-berlin.de
Fax: +49 30 8062 43071
Tel: +49 30 8062 3071 42308

b Physical Chemistry I, University of Bayreuth, Universitätsstr. 30, 95440 Bayreuth, Germany

c Max-Planck-Institut für Metallforschung, Heisenbergstrasse 3, D-70569 Stuttgart, Germany

d Institut für Theoretische und Angewandte Physik, Universität Stuttgart, Pfaffenwaldring 57, D-70569 Stuttgart, Germany

e ESRF, B.P. 220, 38043 Grenoble, France

f Department of Physics, Humboldt University Berlin, Newtonstr. 15, 12489 Berlin, Germany

Abstract

We present an investigation of β-lactoglobulin adsorption onto spherical polyelectrolyte brushes (SPBs) by small angle X-ray scattering (SAXS). The SPB consists of a polystyrene core onto which long chains of poly(styrene sulfonate) are grafted. The amount and the distribution of proteins adsorbed in the brush layer at low ionic strength can be derived from SAXS. The analysis of the SAXS data reveals additionally that some of the protein molecules form aggregates of about six monomers in the adsorbed state. Furthermore, the position and the amount of slightly bound protein can be detected by the combination of the SAXS results and the SPB loading after extensive ultrafiltration. The total amount of adsorbed protein is compared to data derived from isothermal titration calorimetry. The comparison of both sets of data demonstrates that the protein molecules in the inner layers of the spherical polyelectrolyte brush are firmly bound. Proteins located in the outer layers are only weakly bound and can be washed out by prolonged ultrafiltration.

Graphical abstract: Interaction strength between proteins and polyelectrolyte brushes: a small angle X-ray scattering study

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Article information

DOI
https://doi.org/10.1039/C1CP20663J
Article type
Paper
Submitted
08 Mar 2011
Accepted
09 Aug 2011
First published
05 Sep 2011

Phys. Chem. Chem. Phys., 2011,13, 17599-17605

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Interaction strength between proteins and polyelectrolyte brushes: a small angle X-ray scattering study

K. Henzler, B. Haupt, S. Rosenfeldt, L. Harnau, T. Narayanan and M. Ballauff, Phys. Chem. Chem. Phys., 2011, 13, 17599 DOI: 10.1039/C1CP20663J

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