Nucleic acid        X-ray crystallography        via direct selenium derivatization

Lina Lin; Jia Sheng; Zhen Huang

doi:10.1039/C1CS15020K

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Nucleic acid X-ray crystallographyvia direct selenium derivatization

Lina Lin,^a Jia Sheng^a and Zhen Huang*^a

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* Corresponding authors

^a Department of Chemistry, Georgia State University, Atlanta, GA, USA
E-mail: huang@gsu.edu
Fax: +1-404-413-5505
Tel: +1-404-413-5535

Abstract

X-ray crystallography has proven to be an essential tool for structural studies of bio-macromolecules at the atomic level. There are two major bottle-neck problems in the macromolecular crystal structure determination: phasing and crystallization. Although the selenium derivatization is routinely used for solving novel protein structures through the MAD phasing technique, the phase problem is still a critical issue in nucleic acid crystallography. The background and current progress of using direct selenium-derivatization of nucleic acids (SeNA) to solve the phase problem and to facilitate nucleic acid crystallization for X-ray crystallography are summarized in this tutorial review.

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Article information

DOI: https://doi.org/10.1039/C1CS15020K
Article type: Tutorial Review
Submitted: 17 Jan 2011
Accepted: 05 Apr 2011
First published: 13 Jun 2011

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Chem. Soc. Rev., 2011,40, 4591-4602

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Nucleic acid X-ray crystallography via direct selenium derivatization

L. Lin, J. Sheng and Z. Huang, Chem. Soc. Rev., 2011, 40, 4591 DOI: 10.1039/C1CS15020K

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