Issue 10, 2011
From the journal:

Energy & Environmental Science

Application of monoclonal antibodies to investigate plant cell wall deconstruction for biofuels production†

Jaclyn D. DeMartini,abe   Sivakumar Pattathil,ce   Utku Avci,ce   Kaitlyn Szekalski,c   Koushik Mazumder,ce   Michael G. Hahncde  and  Charles E. Wyman*abe  

* Corresponding authors

a Chemical and Environmental Engineering Department, University of California, Riverside, Riverside, CA, USA
E-mail: Charles.wyman@ucr.edu
Fax: +951-781-5790
Tel: +951-781-5703

b Center for Environmental Research and Technology, Bourns College of Engineering, University of California, Riverside, 1084 Columbia Ave, Riverside, CA, USA

c Complex Carbohydrate Research Center, The University of Georgia, 315 Riverbend Rd., Athens, Georgia, USA

d Department of Plant Biology, University of Georgia, Athens, GA, USA

e BESC BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, USA

Abstract

To better understand how hydrothermal pretreatment reduces plant cell wall recalcitrance, we applied a high throughput approach (“glycome profiling”) using a comprehensive suite of plant glycan-directed monoclonal antibodies to monitor structural/extractability changes in Populus biomass. The results of glycome profiling studies were verified by immunolabeling using selected antibodies from the same toolkit. The array of monoclonal antibodies employed in these studies is large enough to monitor changes occurring in most plant cell wall polysaccharides. Results from these techniques demonstrate the sequence of structural changes that occur in plant cell walls during pretreatment-induced deconstruction, namely, the initial disruption of lignin-polysaccharide interactions in concert with a loss of pectins and arabinogalactans; this is followed by significant removal of xylans and xyloglucans. Additionally, this study also suggests that lignin content per se does not affect recalcitrance; instead, the integration of lignin and polysaccharides within cell walls, and their associations with one another, play a larger role.

Graphical abstract: Application of monoclonal antibodies to investigate plant cell wall deconstruction for biofuels production

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Article information

DOI
https://doi.org/10.1039/C1EE02112E
Article type
Paper
Submitted
10 Jul 2011
Accepted
17 Aug 2011
First published
06 Sep 2011

Energy Environ. Sci., 2011,4, 4332-4339

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Application of monoclonal antibodies to investigate plant cell wall deconstruction for biofuels production

J. D. DeMartini, S. Pattathil, U. Avci, K. Szekalski, K. Mazumder, M. G. Hahn and C. E. Wyman, Energy Environ. Sci., 2011, 4, 4332 DOI: 10.1039/C1EE02112E

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