Issue 21, 2012
From the journal:

Lab on a Chip

Microfluidic diagnostic tool for the developing world: contactless impedance flow cytometry

Sam Emaminejad,†*ab   Mehdi Javanmard,†b   Robert W. Duttona  and  Ronald W. Davisb  

* Corresponding authors

a Dept. of Electrical Engineering, Stanford University, Stanford, CA, USA
E-mail: same@stanford.edu (S.E.)

b Stanford Genome Technology Center, Stanford, CA, USA
E-mail: mehdij@stanford.edu (M.J.)

Abstract

In this work, we demonstrate a novel and cost-effective approach to implement a disposable microfluidic contactless impedance cytometer. Conventional methods for single cell impedance cytometry use microfabricated electrodes in direct contact with the buffer to measure changes of its electrical impedance when cells pass through the applied electric field. However, this approach requires expensive microfabrication of electrodes, and also, the fabricated electrodes cannot be reused without thorough and time-consuming cleaning process. Here, we introduce a novel approach to allow for single cell impedance cytometry using electrodes that can be reused, without the need for microfabrication of the electrodes. This disposable device can be potentially inserted onto a printed circuit board (PCB) which has a non-disposable, yet inexpensive, electronic reading apparatus. This significantly reduces the manufacturing costs, making it suitable for low resource settings, such as point-of-care testing in the developing countries.

Graphical abstract: Microfluidic diagnostic tool for the developing world: contactless impedance flow cytometry

About
Cited by
Related
Download options Please wait...

Article information

DOI
https://doi.org/10.1039/C2LC40759K
Article type
Paper
Submitted
05 Jul 2012
Accepted
19 Aug 2012
First published
21 Aug 2012

Lab Chip, 2012,12, 4499-4507

Permissions

Request permissions

Microfluidic diagnostic tool for the developing world: contactless impedance flow cytometry

S. Emaminejad, M. Javanmard, R. W. Dutton and R. W. Davis, Lab Chip, 2012, 12, 4499 DOI: 10.1039/C2LC40759K

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements