Issue 3, 2013
From the journal:

Chemical Science

A near-infrared reversible fluorescent probe for real-time imaging of redox status changes in vivo†

Kehua Xu,a   Mingming Qiang,a   Wen Gao,a   Ruixian Su,a   Na Li,a   Yan Gao,a   Yanxia Xie,a   Fanpeng Konga  and  Bo Tang*a  

* Corresponding authors

a College of Chemistry, Chemical Engineering and Materials Science, Engineering Research Center of Pesticide and Medicine Intermediate Clean Production, Ministry of Education, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Normal University, Jinan 250014, P.R. China
E-mail: tangb@sdnu.edu.cn
Fax: +86 531-8618-0017

Abstract

Alterations of cellular redox status are closely associated with physiological and pathological processes. Glutathione (GSH) and H2O2 should be the most representative redox couple in living cells. However, up to now, there is no way to reversibly detect GSH/H2O2. In this report, a near-infrared (NIR) fluorescent probe (Cy-O-Eb) for monitoring the changes of GSH/H2O2 levels in vivo was developed based on switching on–off a five-membered ring involved in ebselen. This probe could reversibly respond to GSH and H2O2 with high selectivity, sensitivity and mitochondrial targeting. It was successfully used to monitor the changes of redox status during apoptosis and the H2O2 changes at the wound margin in zebrafish larvae. Thus, the probe would provide an ideal tool for monitoring redox status changes and studying molecular events involved in redox regulation.

Graphical abstract: A near-infrared reversible fluorescent probe for real-time imaging of redox status changes in vivo

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Article information

DOI
https://doi.org/10.1039/C2SC22076H
Article type
Edge Article
Submitted
16 Oct 2012
Accepted
09 Dec 2012
First published
14 Dec 2012

Chem. Sci., 2013,4, 1079-1086

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A near-infrared reversible fluorescent probe for real-time imaging of redox status changes in vivo

K. Xu, M. Qiang, W. Gao, R. Su, N. Li, Y. Gao, Y. Xie, F. Kong and B. Tang, Chem. Sci., 2013, 4, 1079 DOI: 10.1039/C2SC22076H

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