Issue 24, 2013
From the journal:

Analyst

Cholesterol determination using protein-templated fluorescent gold nanocluster probes†

Xi Chena  and  Gary A. Baker*a  

* Corresponding authors

a Department of Chemistry, University of Missouri-Columbia, Columbia, MO 65211, USA
E-mail: bakergar@missouri.edu
Tel: +1 573-882-1811

Abstract

We describe the development of a fluorescent biosensor platform for soluble cholesterol based on bovine serum albumin-stabilized gold nanocluster probes co-dissolved with cholesterol oxidase (ChOx) in a surfactant emulsion system. Selective enzymatic oxidation of cholesterol to cholest-4-en-3-one by ChOx produces stoichiometric amounts of H2O2 by-product, generating a quenching response signaling the presence of cholesterol at clinically relevant levels (LOD ∼12 μM).

Graphical abstract: Cholesterol determination using protein-templated fluorescent gold nanocluster probes

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Article information

DOI
https://doi.org/10.1039/C3AN01548C
Article type
Communication
Submitted
15 Aug 2013
Accepted
18 Oct 2013
First published
18 Oct 2013

Analyst, 2013,138, 7299-7302

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Cholesterol determination using protein-templated fluorescent gold nanocluster probes

X. Chen and G. A. Baker, Analyst, 2013, 138, 7299 DOI: 10.1039/C3AN01548C

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