RF sputtered tin oxide (SnO₂) thin film matrix has been efficiently exploited for the detection of uric acid. The deposition parameters for SnO₂ thin film have been optimized to yield better electrocatalytic properties. A correlation between its electrocatalytic properties with the structural and electrical properties has been made. SnO₂ thin film prepared under optimized growth parameter (70% argon in reactive gas ambient of Ar and O₂) exhibits higher mobility of charge carrier and high carrier concentration thereby resulting in enhanced charge transfer characteristics. High surface coverage of uricase onto SnO₂ thin films (4.28 × 10⁻⁴ mole cm⁻²), low value of Michaelis–Menten constant (k_m) 0.18 mM, good linearity in detection of uric acid in the range 0.05–1.00 mM and a fast response of 5 s are attractive features of prepared SnO₂ thin film based bioelectrodes for efficient detection of uric acid. The nanoporous and rough surface morphology of SnO₂ thin film besides its high carrier mobility in comparison to that of ITO is responsible for the obtained enhanced sensitivity (∼700 μA mM⁻¹) and improved sensing response characteristics towards uric acid.