Issue 10, 2015

Aptamer-based colorimetric biosensing of abrin using catalytic gold nanoparticles

Abstract

In this study we propose a simple and sensitive colorimetric aptasensor for the quantitative analysis of abrin by using catalytic AuNPs for the first time. AuNPs possess the peroxidase-like activity that can catalyse 3,3,5,5-tetramethylbenzidine (TMB) in the presence of H2O2, leading to color change of the solution. It is interesting to find that the peroxidase-like activity of AuNPs can be improved by surface activation with a target-specific aptamer. However, with a target molecule, the aptamer is desorbed from the AuNPs surface, resulting in a decrease of the catalytic abilities of AuNPs. The color change of the solution is relevant to the target concentration, and this can be judged by the naked eye and monitored by using a UV-vis spectrometer. The linear range for the current analytical system was from 0.2 nM to 17.5 nM. The corresponding limit of detection (LOD) was 0.05 nM. Some other proteins such as thrombin (Th), glucose oxidase (GOx), and bovine serum albumin (BSA) all had a negligible effect on the determination of abrin. Furthermore, several practical samples spiked with abrin were analyzed using the proposed method with excellent recoveries. This aptamer-based colorimetric biosensor is superior to other conventional methods owing to its simplicity, low cost, and high sensitivity.

Graphical abstract: Aptamer-based colorimetric biosensing of abrin using catalytic gold nanoparticles

Supplementary files

Article information

Article type
Paper
Submitted
18 Jan 2015
Accepted
19 Mar 2015
First published
20 Mar 2015

Analyst, 2015,140, 3581-3586

Author version available

Aptamer-based colorimetric biosensing of abrin using catalytic gold nanoparticles

J. Hu, P. Ni, H. Dai, Y. Sun, Y. Wang, S. Jiang and Z. Li, Analyst, 2015, 140, 3581 DOI: 10.1039/C5AN00107B

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements