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Both geometric morphometric and microsatellite data consistently support the differentiation of the Apis mellifera M evolutionary branch

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Abstract

Traditional morphometrics, allozymes, and mitochondrial data have supported a close relationship between the M branch subspecies A. m. iberiensis and the North African subspecies (A branch). However, studies using nuclear DNA markers have revealed a clear distinction between the latter and the two European M branch subspecies. In help resolve this paradox, we analyzed 663 colonies from six European and African subspecies. A geometric morphometrics approach was applied to the analysis of wing shape, and the results were compared with data of six microsatellite loci. Both data sets were found to be highly consistent and corroborated a marked divergence of West European subspecies from North African ones. This supports the hypothesis that the presence of the African lineage mitotype in Iberian honey bee populations is likely the consequence of secondary introductions, with a minimal African influence within the current Iberian genetic background. Wing geometric morphometrics appears more appropriate than mitochondrial DNA analysis or traditional morphometrics in the screening and identification of the Africanization process.

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Authors and Affiliations

  1. Department of Genetics, Physical Anthropology and Animal Physiology, Faculty of Science and Technology, University of the Basque Country, 48940, Leioa, Spain

    Irati Miguel, Mikel Iriondo, Carmen Manzano & Andone Estonba

  2. Muséum National d’Histoire Naturelle, CNRS UMR 7205 and UMS 2700, Plate-Forme Morphométrie, CP 50, 45 rue Buffon, 75005, Paris, France

    Michel Baylac

  3. Laboratoire Évolution, Génomes et Spéciation, CNRS, avenue de la Terrasse, 91198, Gif-sur-Yvette Cedex, France

    Lionel Garnery

  4. UFR des sciences, Université de Versailles Saint-Quentin-en-Yvelines, 45 avenue des États-Unis, 78035, Versailles, France

    Lionel Garnery

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  1. Irati Miguel
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  2. Michel Baylac
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  3. Mikel Iriondo
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Correspondence to Irati Miguel.

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Manuscript editor: Marina Meixner

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Miguel, I., Baylac, M., Iriondo, M. et al. Both geometric morphometric and microsatellite data consistently support the differentiation of the Apis mellifera M evolutionary branch. Apidologie 42, 150–161 (2011). https://doi.org/10.1051/apido/2010048

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