Subscribe to RSS
DOI: 10.1055/s-0034-1382371
Antitumor metabolites from the Red Sea sponge Spheciospongia vagabunda
Several extracts of Egyptian marine organisms from the Red Sea were screened for their anticancer activity using Sulpho-Rhodamine-B (SRB) assay. The results indicated that the extracts of the sponge Spheciospongia vagabunda possessed anticancer activity against HepG2 (liver cancer cell line) and MCF-7 (breast cancer cell line) with IC50s of 28.1 and 19.7 µg/ml respectively. Bioactivity-guided fractionation of S. vagabunda methanolic extract led to the isolation of three new ceramides; N-[((2S,3S,4R)-1,3,4-trihydroxytetradecan-2-yl] tridecanamide (1), (R)-2'-hydroxy-N-[(2S,3S,4R)-1,3,4-trihydroxypentacosan-2-yl] octadecanamide (2) and (R,Z)-2'-hydroxy-N-[(2S,3S,4R)-1,3,4- trihydroxytricosan-2-yl) nonadec-10-enamide (3). Compounds 1, 2 and 3 displayed high potential cytotoxicity against HepG2 (IC50s 48.3, 17.2, and 14.5 µg/ml, respectively) and MCF-7 (45.6, 18.7, and 20.3 µg/ml, respectively) compared to cisplatin as control drug.