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Genetic transformation of green-colored cotton

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Summary

This study reports an Agrobacterium-mediated transformation of green-colored cotton (Gossypium hirsutum L.). A tissue culture procedure was optimized to induce callus formation from hypocotyl explants and subsequent differentiation into the embryogenic type. Callus formation could be induced by growing explants on Murashige and Skoog medium containing 2,4-dichlorophenoxyacetic acid and kinetin. Among the four genotypes studied, embryogenic calli and plant regeneration were observed only in var. G9803. Agrobacterium-mediated transformation of G9803 with the fiber-specific expansin gene GhExpl was achieved based on the establishment of these tissue culture methods. A total of 32 individual regenerants resistant to kanamycin were generated within 7 mo., with a transformation frequency of 17.8%. Transformation was confirmed by Southern blot analysis and RT-PCR. These results represent the first step towards genetic manipulation of the colors and fiber quality of green-colored cottons by biotechnology.

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Author notes

  1. Zhi-Yong Wang

    Present address: Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, 100093, Beijing, P.R. China

Authors and Affiliations

  1. Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, 100093, Beijing, P.R. China

    Sheng-Wei Zhu, Jing-San Sun, Hai-Hua Wang & Xiao-Min Luo

  2. Institute of Microbiology, Chinese Academy of Sciences, 100080, Beijing, P.R. China

    Peng Gao & Gui-Xian Xia

  3. Institute of Naturally Colored Cotton, 830016, Xinjiang, P.R. China

    Ming-Yu Jiao

Authors
  1. Sheng-Wei Zhu
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  2. Peng Gao
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  3. Jing-San Sun
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  4. Hai-Hua Wang
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  5. Xiao-Min Luo
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  6. Ming-Yu Jiao
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  7. Zhi-Yong Wang
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  8. Gui-Xian Xia
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Correspondence to Gui-Xian Xia.

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These authors contributed equally to this work

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Zhu, SW., Gao, P., Sun, JS. et al. Genetic transformation of green-colored cotton. In Vitro Cell.Dev.Biol.-Plant 42, 439–444 (2006). https://doi.org/10.1079/IVP2006777

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  • DOI: https://doi.org/10.1079/IVP2006777

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