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Volume 49, Issue 2

Characterization of the anaerobic propionate-degrading syntrophs gen. nov., sp. nov. and Free

Abstract

A strain of anaerobic, syntrophic, propionate-oxidizing bacteria, strain LYP(= OCM 661; T = type strain), was isolated and proposed as representative a new genus and new species, gen. nov., sp. nov. The strain was enriched from an anaerobic digestor and isolated. Initial isolation was as a monoxenic propionate-degrading co-culture containing JF-1as an H- and formate-using partner. Later, an axenic culture was obtained by using crotonate as the catabolic substrate. The previously described propionate-degrading syntrophs of the genus also grow in co-culture with methanogens such as , forming acetate, CO and methane from propionate. However, differs by producing less methane and more acetate; in addition, it forms small amounts of butyrate. and grew within similar ranges of pH, temperature and salinity, but they differed significantly in substrate ranges and catabolic products. Unlike grew axenically on crotonate, although very slowly. Co-cultures of grew on propionat and grew slowly on crotonate or butyrate. and grow on propionate plus sulfate, whereas did not. Comparisons of 16S rDNA genes indicated that is most closely related to , and is more distantly related to

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Keyword(s): anaerobic digestor , propionate-oxidizing bacteria and Smithella propionica
© 1999, International Union of Microbiological Societies
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1999-04-01
2024-04-25
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Characterization of the anaerobic propionate-degrading syntrophs Smithella propionica gen. nov., sp. nov. and Syntrophobacter wolinii
Int J Syst Evol Microbiol 49, 545 (1999); https://doi.org/10.1099/00207713-49-2-545
/content/journal/ijsem/10.1099/00207713-49-2-545
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