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Volume 62, Issue Pt_8

sp. nov., isolated from the marine sponge collected from the Red Sea Free

Abstract

A Gram-negative, catalase- and oxidase-positive, non-sporulating, rod-shaped and slightly halophilic bacterial strain, designated UST090418-1611, was isolated from the marine sponge collected from the Red Sea coast of Saudi Arabia. Phylogenetic trees based on the 16S rRNA gene sequence placed strain UST090418-1611 in the family with the closest relationship to the genus . The 16S rRNA gene sequence similarity between the strain and the type strains of recognized species ranged from 92.9 to 98.3 %. Although strain UST090418-1611 shared high 16S rRNA gene sequence similarity with CN46, CN74 and R65 (98.3, 97.4 and 97.3 %, respectively), the relatedness of the strain to these three strains in DNA–DNA hybridization was only 58, 56 and 33 %, respectively, supporting the novelty of the strain. In contrast to most strains in the genus , strain UST090418-1611 tolerated only 6 % (w/v) NaCl, and optimal growth occurred at 2.0 % (w/v) NaCl, pH 7.0–8.0 and 28–36 °C. The predominant cellular fatty acids were C 3-OH, C, C and summed feature 3 (Cω6 and/or Cω7). The genomic DNA G+C content was 57.1 mol%. Based on the physiological, phylogenetic and chemotaxonomic characteristics presented in this study, we suggest that the strain represents a novel species in the genus , for which the name sp. nov. is proposed, with UST090418-1611 ( = JCM 17469  = NRRL B-59512) as the type strain.

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Funding
This study was supported by the:
  • KAUST (Award SA-C0040/UK-C0016)
© 2012 IUMS
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2012-08-01
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Marinobacter xestospongiae sp. nov., isolated from the marine sponge Xestospongia testudinaria collected from the Red Sea
Int J Syst Evol Microbiol 62, 1980 (2012); https://doi.org/10.1099/ijs.0.028811-0
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