Fluorescence excitation-emission matrices of selected biological materials

M. Wlodarski; M. Kaliszewski; M. Kwasny; K. Kopczynski; Z. Zawadzki; Z. Mierczyk; J. Mlynczak; E. Trafny; M. Szpakowska

doi:10.1117/12.687872

13 October 2006 Fluorescence excitation-emission matrices of selected biological materials

M. Wlodarski, M. Kaliszewski, M. Kwasny, K. Kopczynski, Z. Zawadzki, Z. Mierczyk, J. Mlynczak, E. Trafny, M. Szpakowska

Proceedings Volume 6398, Optically Based Biological and Chemical Detection for Defence III; 639806 (2006) https://doi.org/10.1117/12.687872
Event: Optics/Photonics in Security and Defence, 2006, Stockholm, Sweden

Abstract

EX-EM fluorescence matrices from suspensions of 11 vegetative bacteria, 5 spores, and 14 interferents (fungi, pollens) were measured and cross-sections of the selected bacteria were calculated. The changes of fluorescence characteristics of vegetative cells during their sporulation and starvation to death as well as spores during germination are shown. Influence of culture media on emission spectra and rate of spores formation under starving conditions was examined. Analysis of the measured fluorescence characteristics shows that double- or multi-wavelenghts excitation can make it possible to distinguish between particular groups of biological material, i.e., spores, vegetative cells, proteins, and interferents.

Citation Download Citation

M. Wlodarski, M. Kaliszewski, M. Kwasny, K. Kopczynski, Z. Zawadzki, Z. Mierczyk, J. Mlynczak, E. Trafny, and M. Szpakowska "Fluorescence excitation-emission matrices of selected biological materials", Proc. SPIE 6398, Optically Based Biological and Chemical Detection for Defence III, 639806 (13 October 2006); https://doi.org/10.1117/12.687872

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