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Enzymatic hydrolysis of keratin-containing stock for obtaining protein hydrolysates

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Abstract

Optimization of the process of enzymatic hydrolysis of keratin-containing stock aimed at obtaining hydrolysates of high biological value has been performed. The increasing of the stock/water weight ratio, the amount of the alkaline protease preparation from Acremonium chrysogenium added and the temperature of the reaction mixture resulted in an increase in the yield and antioxidant capacity of hydrolysis products. The molecular masses of soluble products obtained under optimal hydrolysis conditions ranged from 3.55 to 3.60 kDa. High antioxidant capacity, 100% bioavailability and a well-balanced amino acid composition was characteristic of the hydrolysis products.

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Authors and Affiliations

  1. Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, 119071, Russia

    I. V. Nikolaev, I. D. Keruchen’ko, E. V. Stepanova, A. D. Satrutdinov, V. P. Khotchenkov, V. G. Volik & O. V. Koroleva

  2. Faculty of Chemistry, Moscow State University, Moscow, 119991, Russia

    N. L. Eremeev & A. P. Sinitsyn

  3. All-Russian Research Institute of Poultry-Processing Industry, p/o Rzhavki, Moscow oblast, 141552, Russia

    S. V. Zinov’ev & D. Yu. Ismailova

  4. All-Russian Research Institute of Food Biotechnology, Moscow, 111033, Russia

    N. V. Tsurikova

Authors
  1. N. L. Eremeev
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  2. I. V. Nikolaev
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  3. I. D. Keruchen’ko
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  4. E. V. Stepanova
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  5. A. D. Satrutdinov
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  6. S. V. Zinov’ev
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  8. V. P. Khotchenkov
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  9. N. V. Tsurikova
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  10. A. P. Sinitsyn
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  11. V. G. Volik
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  12. O. V. Koroleva
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Correspondence to N. L. Eremeev.

Additional information

Original Russian Text © N.L. Eremeev, I.V. Nikolaev, I.D. Keruchen’ko, E.V. Stepanova, A.D. Satrutdinov, S.V. Zinov’ev, D.Yu. Ismailova, V.P. Khotchenkov, N.V. Tsurikova, A.P. Sinitsyn, V.G. Volik, O.V. Koroleva, 2009, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2009, Vol. 45, No. 6, pp. 717–724.

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Eremeev, N.L., Nikolaev, I.V., Keruchen’ko, I.D. et al. Enzymatic hydrolysis of keratin-containing stock for obtaining protein hydrolysates. Appl Biochem Microbiol 45, 648–655 (2009). https://doi.org/10.1134/S0003683809060131

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  • DOI: https://doi.org/10.1134/S0003683809060131

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