Abstract
Lipogenic activity and fatty acid composition of two strains of Geomyces pannorum were studied in the course of fungal growth. The strains were isolated from an Arctic cryopeg lens (VKM FW-2241) and from Central Russia (VKM F-3808). The adaptive reactions in both strains towards the temperature decreasing to 2°C involved intensification of the fatty acid desaturation. The degree of lipid unsaturation increased mainly due to a higher amount of α-linolenic acid (α-C18:3) especially in the case of strain VKM FW-2241. Elevated NaCl concentration in the medium enhanced the level of linoleic acid (C18:2) which apparently played a specific role in osmoprotection. Strain VKM FW-2241 was more tolerant to increased salinity than strain VKM F-3808. Almost complete inhibition of the growth of strains VKM F-3808 and VKM FW-2241 occurred at salinity of 10 and 20%, respectively; however, the viability of the strains was not affected. Under the combined effect of high salinity and hypothermia, the ratio between C18:2 and α-C18:3 acids was intermediate, indicating that these acids were involved in two adaptation mechanisms. The inhibition of fungal growth under stress was found to result in lipid overproduction. An increased pool of energy-rich lipids in fungi possibly contributes to their strategy of cell survival.
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Russell, N.J., Harrisson, P., Johnston, I.A., Jaenicke, R., Zuber, M., Franks, F., and Wynn-Williams, D., Cold Adaptation of Microorganisms [and Discussion], Philos. Trans. R. Soc. Lond. B: Biol. Sci., 1990, vol. 326, pp. 595–611.
Russel, H.J., Evans, P.J., Tersiig, P.F., Helemons, J., Verheeil, A., and Abee, T., Membrane as a Target for Stress Adaptation, Int. J. Food Microbiol, 1995, vol. 28, pp. 255–261.
Chintalapati, S., Kiran, M.D., and Shivaji, S., Role of Membrane Lipid Fatty Acids in Cold Adaptation, Cell. Mol. Biol., 2004, vol. 50, pp. 631–642.
Chatterje, M.T., Khalawan, S.A., and Curran, B.P.G., Cellular Lipid Composition Influences Stress Activation of the Yeast General Stress Response Element (STRE), Microbiology (UK), 2000, vol. 146, pp. 877–884.
Guillot, A., Obis, D., and Mistou M.-Y., Fatty Acid Membrane Composition and Activation of Glycine-Betaine Transport in Lactococcus lactis Subjected to Osmotic Stress, Int. J. Food Microbiol., 2000, vol. 55, no. 1, pp. 47–51.
Guerzoni, M.E., Lanciotti, R., and Cocconcelli, R.S., Alteration in Cellular Fatty Acid Composition as a Response to Salt, Acid, Oxidative and Thermal Stresses in Lactobacillus helveticus, Microbiology (UK), 2001, vol. 147, pp. 2255–2264.
Istokovics, A., Morita, N., Izumi, K., Hoshino, T., Jumoto, J., Davada, M.T., Jshizaki, K., and Okuyama, H., Neutral Lipids, Phospholipids, and a Betaine Lipid of the Snow Mold Fungus Microdochium nivale, Can. J. Microbiol., 1998, vol. 44, pp. 1051–1059.
Manocha, M.S. and Campbell, C.D., The Effect of Growth Temperature on the Fatty Acid Composition of Thamnidium elegans Link, Can. J. Microbiol., 1978, vol. 24, pp. 670–674.
Microbial lipids, Ratledge, C. and Wilkinson, S.G., Eds., London: Academic, 1989–1990, vol. 1–2.
Konova, I.V., Characterization of Actinomycete Lipids, Biol. Nauki, 1983, no. 8, pp. 5–17.
Batrakov, S.G., Konova, I.V., Sheichenko, V.I., Esipov, S.E., Galanina, L.A., and Istratova, L.N., Unusual Fatty Acid Composition of Cerebrosides from the Filamentous Soil Fungus Mortierella alpina, Chem. Phys. Lipids, 2002, vol. 117, pp. 45–51.
Goncharova, O.V., Konova, I.V., and Biryuzova, V.I., Biochemical and Structural Features of Blakeslea trispora Dependent on Medium Composition, Mikrobiologiya, 1996, vol. 65, no. 1, pp. 54–59 [Microbiology (Engl. Transl.), vol. 65, no. 1, pp. 47–51].
Batrakov, S.G., Konova, I.V., Sheichenko, V.I., Esipov, S.E., Galanina, L.A., Istratova, L.N., and Sergeeva, Ya.E., Lipids of the Zygomycete Absidia corymbifera F-965, Phytochemistry, 2004, vol. 65, pp. 1239–1246.
Ozerskaya, S., Ivanushkina, N., Kochkina, G., Fattakhova, R., and Gilichinsky, D., Mycelial Fungi from Cryopegs, Int. J. Astrobiology, 2004, vol. 3, no. 4, pp. 327–331.
Kochkina, G.A., Ivanushkina, N.E., Akimov, V.N., Gilichinskii, D.A., and Ozerskaya, S.M., Halo- and Psychrotolerant Geomyces Fungi from Arctic Cryopegs and Marine Deposits, Mikrobiologiya, 2007, vol. 76, no. 1, pp. 39–47 [Microbiology (Engl. Transl.), vol. 76, no. 1, pp. 31–38].
Keits, M., Techniques of Lipidology: Isolation, Analysis, and Identification of Lipids, Amsterdam: Elsevier, 1972 [Russ. Transl. Moscow: Mir, 1975].
Robinson, C.H., Cold Adaptation in Arctic and Antarctic Fungi, New Phytol., 2001, vol. 151, pp. 341–353.
Arzumanyan, V.G., Voronina, N.A., Geidebrekht, O.V., Shelemekh, O.V., Plakunov, V.K., and Belyaev, S.S., Antagonistic Interactions between Stress Factors during the Growth of Microorganisms under Conditions Simulating the Parameters of Their Natural Ecotopes, Mikrobiologiya, 2002, vol. 71, no. 2, pp. 160–165 [Microbiology (Engl. Transl.), vol. 71, no. 2, pp. 133–138].
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Original Russian Text © I.V. Konova, Ya.E. Sergeeva, L.A. Galanina, G.A. Kochkina, N.E. Ivanushkina, S.M. Ozerskaya, 2009, published in Mikrobiologiya, 2009, Vol. 78, No. 1, pp. 52–58.
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Konova, I.V., Sergeeva, Y.E., Galanina, L.A. et al. Lipid synthesis by Geomyces pannorum under the impact of stress factors. Microbiology 78, 42–47 (2009). https://doi.org/10.1134/S0026261709010068
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DOI: https://doi.org/10.1134/S0026261709010068