Abstract
Algalogically pure cultures were isolated from different aquatic ecosystems and identified as representatives of green algae, euglena algae, and cyanobacteria. Isolated cultures and collection strains of microalgae were screened for productivity. Induced mutagenesis (exposure to UV radiation, 254 nm, 40 Erg/mm2) and selection yielded a mutant strain C-2m2 of Chlorella pyrenoidosa notable for a high activity of lipid biosynthesis and accumulation. Optimization of its culture conditions was conducted. It was found that the active lipid accumulation by the cells of C. pyrenoidosa strain C-2m2 occurred when nitrogen concentration in the medium was tenfold reduced (to the level of 0.004 g/L) at an illuminance of 4 klx.
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Strebkov, D.S., Ross, M.Yu., and Shchekochikhin, Yu.M., Energy use of biomass for the production of algae biofuels, Mater. VI mezhd. nauchno-tekhnicheskoi konf. “Energoobespechenie i energosberezhenie v sel’skom khozyaistve” (Proc. VI Int. Science and Technology Conf. “Energy Supply and Energy Saving in Agriculture”), Moscow: Vseros. Inst. Electrific. S. Kh., 2008, part 4, pp. 408–415.
Schenk, P.M., Thomas-Hall, S.R., Stephens, E., Marx, U., Mussgnug, J., Posten, C., Kruse, O., and Hankamer, B., Second generation biofuels: high-efficiency microalgae for biodiesel production, BioEnergy Res., 2008, vol. 1, pp. 20–43.
Griffiths, M. and Harrison, S., Lipid productivity as a key characteristic for choosing algal species for biodiesel production, J. Appl. Phycol., 2009, vol. 21, pp. 493–507.
Milogradova, E.I. and Muzzafarov, A.M., Mass cultivation of chlorella in Uzbekistan and its use in the economy, Izuchenie intensivnoi kul’tury vodoroslei, Tret’e koord. soveshch. po probleme 9.9 SEV (The 3rd Coord. Meet., iss. 9.9 CMEA, “Study of Intense Algal Culture”), Prague, 1965, pp. 106–114.
Tamiya, H., Morimora, Y., Yokota, M., and Kunieda, R., Mode of nuclear division in synchronous culture of Chlorella: comparison of various methods of synchronization, Plant Cell Physiol., 1961, vol. 2, pp. 383–403.
Greenspan, P., Mayer, E.P., and Fowler, S.D., Nile red: a selective fluorescent stain for intracellular lipid droplets, J. Cell Biol., 1985, vol. 100, pp. 965–973.
Vaulina, E.N., Anikeeva, I.D., and Kogan, I.G., Indutsirovannyi mutagenez i selektsiya khlorelly (Chlorella Induced Mutagenesis and Breeding), Moscow: Nauka, 1978.
Ensminger, P.A. and Schafer, E., Blue and ultraviolet-B light photoreceptors in parsley cells, Photochem. Photobiol., 1992, vol. 55, pp. 437–447.
Kalacheva, G.S. and Trubachev, I.N., Lipids of hydrogen bacteria, Khemosintez v nepreryvnoi kul’ture (Chemosynthesis in Continuous Culture), Terskov, I.A., Ed., Novosibirsk: Nauka, 1978, pp. 89–96.
Yur’eva, M.I., Temnykh, A.A., Gostrenko, L.M., and Akulin, V.I., Fatty acid composition of alga Porphyridium cruentum lipids depending on the culture growth phase, Biol. Morya (Vladivostok), 1984, no. 6, pp. 45–48.
Orcutt, D. and Patterson, G., Effect of light intensity upon lipid composition of Nitzschia closterium (Cylindrotheca fusiformis), Lipids, 1974, vol. 9, pp. 1000–1003.
Henderson, R.J. and Sargent, J.R., Lipid composition and biosynthesis in ageing cultures of the marine cryptomonad Chroomonas salina, Phytochemistry, 1989, vol. 28, pp. 1355–1361.
Merzlyak, M., Chivkunova, O., Gorelova, O., Reshetnikova, I., Solovchenko, A., Khozin-Goldberg, I., and Cohen, Z., Effect of nitrogen starvation on optical properties, pigments, and arachidonic acid content of the unicellular green alga Parietochloris incisa (Trebouxiophyceae, Chlorophyta), J. Phycol., 2007, vol. 43, pp. 833–843.
Hu, Q., Sommerfeld, M., Jarvis, E., Ghirardi, M., Posewitz, M., Seibert, M., and Darzins, A., Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances, Plant J., 2008, vol. 54, pp. 621–639.
Gardner, R., Peters, P., Peyton, B., and Cooksey, K.E., Medium pH and nitrate concentration effects on accumulation of triacylglycerol in two members of the Chlorophyta, J. Appl. Phycol., 2011, vol. 23, pp. 1005–1016.
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Original Russian Text © B.K. Zayadan, S. Purton, A.K. Sadvakasova, A.A. Userbaeva, K. Bolatkhan, 2014, published in Fiziologiya Rastenii, 2014, Vol. 61, No. 1, pp. 135–142.
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Zayadan, B.K., Purton, S., Sadvakasova, A.K. et al. Isolation, mutagenesis, and optimization of cultivation conditions of microalgal strains for biodiesel production. Russ J Plant Physiol 61, 124–130 (2014). https://doi.org/10.1134/S102144371401018X
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DOI: https://doi.org/10.1134/S102144371401018X