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Coupling recovery strategy of cellulase in hydrolyzate after hydrolysis with Tannin flocculation and PEG desorption

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Abstract

Through determination of cellulase in terms of protein content in the hydrolyzate after hydrolysis, it was found that 50.15–67.21% of the originally added protein was still remained in the hydrolyzate. Tannin flocculation followed by PEG desorption was coupled to recover this portion of cellulase. About 94.99% of the protein in the hydrolyzate was precipitated into Tannin-cellulase compound under tannin concentration of 5% (w/v) with pH 5.0 and NaCl concentration of 1.0%. In order to desorb cellulase from Tannin-cellulase compound, PEG 6000 was employed as a desorbent. The recovered enzyme activity reached 133.25% when the PEG concentration was 1.5% (w/v). This, to some extent, indicated that PEG was also an effective activator of cellulase. The article is published in the original.

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Authors and Affiliations

  1. State Key Lab of Biochemical Engineering, Institute of Process Engineering, CAS, Beijing, 100080, China

    J. Xu & H. Chen

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  1. J. Xu
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  2. H. Chen
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Correspondence to J. Xu.

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Published in Russian in Prikladnaya Biokhimiya i Mikrobiologiya, 2009, Vol. 45, No. 3, pp. 344–347.

The article is published in the original.

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Xu, J., Chen, H. Coupling recovery strategy of cellulase in hydrolyzate after hydrolysis with Tannin flocculation and PEG desorption. Appl Biochem Microbiol 45, 309–312 (2009). https://doi.org/10.1134/S0003683809030120

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  • DOI: https://doi.org/10.1134/S0003683809030120

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