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Strain improvement for enhanced production of cellulase in Trichoderma viride

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Abstract

The filamentous fungi Trichoderma species produce extracellular cellulase. The current study was carried out to obtain an industrial strain with hyperproduction of cellulase. The wild-type strain, Trichoderma viride TL-124, was subjected to successive mutagenic treatments with UV irradiation, low-energy ion beam implantation, atmospheric pressure non-equilibrium discharge plasma (APNEDP), and N-methyl-N′-nitro-N-nitrosoguanidine to generate about 3000 mutants. Among these mutants, T. viride N879 strain exhibited the greatest relevant activity: 2.38-fold filter paper activity and 2.61-fold carboxymethyl cellulase, 2.18-fold β-glucosidase, and 2.27-fold cellobiohydrolase activities, compared with the respective wild-type activities, under solid-state fermentation using the inexpensive raw material wheat straw as a substrate. This work represents the first application of APNEDP in eukaryotic microorganisms.

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References

  1. Jarvis, M., Science, 2003, vol. 426, no. 6967, pp. 611–612.

    CAS  Google Scholar 

  2. Zhang, Y.H.P. and Lynd, L.R., Biotechnol. Bioeng., 2004, vol. 88, no. 7, pp. 797–824.

    Article  CAS  PubMed  Google Scholar 

  3. Adsul, M.G., Bastawde, K.B., Varma, A.J., and Gokhale, D.V., Bioresource Technol., 2007, vol. 98, no. 7, pp. 1467–1473.

    Article  CAS  Google Scholar 

  4. Gomes, I., Gomes, J., Steiner, W., and Esterbauer, H., Appl. Microbiol. Biotechnol., 1992, vol. 36, no. 5, pp. 701–707.

    Article  CAS  Google Scholar 

  5. Henrissat, B., Cellulose, 1994, vol. 1, no. 3, pp. 169–196.

    Article  CAS  Google Scholar 

  6. Zhang, Y.H.P., Himmel, M.E., and Mielenz, J.R., Biotechnol. Adv., 2006, vol. 24, no. 5, pp. 452–481.

    Article  CAS  Google Scholar 

  7. Martins, L.F., Kolling, D., Camassola M., Dillon A.J.P., and Ramos, L.P., Bioresource Technol., 2008, vol. 99, no. 5, pp. 1417–1424.

    Article  CAS  Google Scholar 

  8. Ahamed, A. and Vermette, P., Biochem. Eng. J., 2008, vol. 40, pp. 399–407.

    Article  CAS  Google Scholar 

  9. Zaldivar, J., Nielsen, J., and Olsson, L., Appl. Microbiol. Biotechnol., 2001, vol. 56, no. 1–2, pp. 17–34.

    Article  CAS  PubMed  Google Scholar 

  10. Anwar, M.N., Suto, M., and Tomita, F., Appl. Microbiol. Biotechnol., 1996, vol. 45, no., pp. 684–647.

    Article  CAS  Google Scholar 

  11. Szengyel, Z., Zacchi, G., Varga, A., and Reczey, K., Appl. Biochem. Biotechnol., 2000, vol. 84–86, no. 1–9, pp. 679–691.

    Article  PubMed  Google Scholar 

  12. Xia, L.M. and Shen, X.L., Bioresource Technol., 2004, vol. 91, no. 3, pp. 259–262.

    Article  CAS  Google Scholar 

  13. Chand, P., Aruna, A., Maqsood, A.M., and Rao, L.V., J. Appl. Microbiol., 2005, vol. 98, no. 2, pp. 318–323.

    Article  CAS  PubMed  Google Scholar 

  14. Su, C.X., Zhou W., Fan, Y.H., Wang, L., Zhao, S.G., and Yu, Z.L., J. Ind. Microbiol. Biotechnol., 2006, vol. 33, no. 12, pp. 1037–1042.

    Article  CAS  PubMed  Google Scholar 

  15. Gu, S.B., Yao, J.M., Yuan, Q.P., Xue, P.J., Zheng, Z.M., Wang, L., and Yu, Z.L., Appl. Microbiol. Biotechnol., 2006, vol. 72, no. 3, pp. 456–461.

    Article  CAS  PubMed  Google Scholar 

  16. Wang, L.Y., Huang, Z.L., Li, G., Zhao, H.X., Xing, X.H., Sun, W.T., Li, H.P., Gou, Z.X., and Bao, C.Y., J. Appl. Microbiol., 2010, vol. 108, no. 3, pp. 851–858.

    Article  CAS  PubMed  Google Scholar 

  17. Tao, L., Dong, H.J., Chen, X., Chen, S.F., and Wang, T.H., Appl. Microbiol. Biotechnol., 2008, vol. 80, no. 4, pp. 573–578.

    Article  CAS  PubMed  Google Scholar 

  18. Gao, J.M., Weng, H.B., Zhu, D.H., Yuan, M.X., Guan, F.X., and Xi, Y., Bioresourse Technol., 2008, vol. 99, no. 16, pp. 7623–7629.

    Article  CAS  Google Scholar 

  19. Penttila, M, Nevalainen., H., Ratto, M., Salminen, E., and Knowles, J., Gene, 1987, vol. 61, no. 2, pp. 155–164.

    Article  CAS  PubMed  Google Scholar 

  20. Rubinder, K., Chadha, B.S., Sing, N., Saini, H.S., and Singh, S., J. Ind. Microbiol. Biotechnol., 2002, vol. 29, no. 2, pp. 70–74.

    Article  CAS  PubMed  Google Scholar 

  21. Zengliang, Yu. et al., People's Republic of China Patent No. ZL93103361.6, 2000.

  22. Latifian, M., Hamidi-Esfahani, Z., and Barzegar, M., Bioresource Technol., 2007, vol. 98, no. 18, pp. 3634–3637.

    Article  CAS  Google Scholar 

  23. Ghose, T.K., Pure Appl. Chem., 1987, vol. 59, no. 2, pp. 257–268.

    Article  CAS  Google Scholar 

  24. Miller, G.L., Anal. Chem., 1959, vol. 31, no. 3, pp. 426–428.

    Article  CAS  Google Scholar 

  25. Teather, R.M. and Wood P.J., Appl. Environ. Microb., 1982, vol. 43, no. 4, pp. 777–780.

    CAS  Google Scholar 

  26. Prabavathy, V.R., Mathivanan, N., Sagadevan, E., Murugesan, K., and Lalithakumari, D., Enzyme Microb. Technol., 2006, vol. 38, no. 5, pp. 719–723.

    Article  CAS  Google Scholar 

  27. Adney, W.S., Mohagheghi, A., Thomas, S.R., and Himmel, M., Am. Chem. Soc., Washington, 1995, pp. 256–271.

  28. Zaia, D.A.M., Zaia, C.T.B.V., and Lichtig, J., Quim. Nova, 1998, vol. 21, no. 6, pp. 787–793.

    CAS  Google Scholar 

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Authors and Affiliations

  1. State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing, 100193, China

    F. Xu, J. Wang & S. Chen

  2. Biorefining Research Initiative and Department of Biology, Lakehead University, Thunder Bay, ON, Canada, P7B 5E1

    W. Qin

  3. Key Laboratory of Ion Beam Bioengineering, Institute of Plasma Physics, Chinese Academy of Sciences, Hefei, Anhui, 230031, China

    Z. Yu

  4. Department of Chemical Engineering, Tsinghua University, Beijing, 100084, China

    H. Zhao & X. Xing

  5. Department of Engineering Physics, Tsinghua University, Beijing, 100084, China

    H. Li

Authors
  1. F. Xu
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  2. J. Wang
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  3. S. Chen
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  4. W. Qin
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  5. Z. Yu
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  6. H. Zhao
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  7. X. Xing
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  8. H. Li
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Correspondence to S. Chen.

Additional information

Published in Russian in Prikladnaya Biokhimiya i Mikrobiologiya, 2011, Vol. 47, No. 1, pp. 61–65.

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Xu, F., Wang, J., Chen, S. et al. Strain improvement for enhanced production of cellulase in Trichoderma viride . Appl Biochem Microbiol 47, 53–58 (2011). https://doi.org/10.1134/S0003683811010212

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  • DOI: https://doi.org/10.1134/S0003683811010212

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