Abstract
A comparative assessment of the contents of total nitrogen, ammonium, nitrates, and fungal and bacterial biomasses and of the activity of nitrogen transformation in fresh casts of Aporrectodea caliginosa and in a soddy-podzolic soil was performed. The total content of nitrogen in the casts was similar to or slightly higher than that in the soil; the content of inorganic forms of nitrogen in the casts was significantly higher than in the soil. The intensities of ammonification, nitrification, and denitrification and the pool of microbial biomass with a predominance of fungi during the 1.5-week-long incubation were also significantly higher in the casts. The activity of nitrogen fixation in the casts was lower than that in the soil. In the course of the incubation, the values of these parameters in the casts became closer to those in the soil. The inhibition of fungi in the casts with cycloheximide resulted in an increasing content of inorganic nitrogen and a higher activity of denitrification. In our opinion, this phenomenon is related to the fact that limitation of the fungal growth decreases the intensity of immobilization of nitrates and the fungal competition with denitrifying bacteria for available carbon. It was supposed that the activation of the fungal growth via application of plant substrates into the soil with a high population density of earthworms could suppress the emission of gaseous nitrogen compounds. The soil processing by earthworms had a positive effect on the soil properties.
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References
E. V. Arinushkina, Handbook on the Chemical Analysis of Soils (Mosk. Gos. Univ., Moscow, 1962) [in Russian].
N. P. Bityutskii, I. N. Lapshina, E. I. Lukina, et al., “Role of Earthworms in Mineralization of Organic Nitrogen Compounds in Soil,” Pochvovedenie, No. 10, 1242–1250 (2002) [Eur. Soil Sci. 35 (10), 1100–1108 (2002)].
B. A. Byzov, Zoomicrobial Interactions in the Soil (Geos, Moscow, 2005) [in Russian].
D. T. Zvyagintsev, Methods of Soil Microbiology and Biochemistry (Mosk. Gos. Univ., Moscow, 1991) [in Russian].
P. A. Kozhevin, Microbial Populations in the Nature (Mosk. Gos. Univ., Moscow, 1989) [in Russian].
V. N. Kudeyarov, “Colorimetric Determination of Ammonium Nitrogen in Soils and Plants by the Phenol Method,” Agrokhimiya, No. 5, 146–150 (1965).
A. V. Kurakov, Extended Abstract of Doctoral Dissertation in Biology (Moscow, 2003) [in Russian].
A. V. Kurakov, S. A. Kharin, T. Yu. Nechitailo, et al., “Modification in the Abundance, Biomass, and Composition of Fungi during the Passage of Soil through the Gastrointestinal Tract of the Earthworm Aporrectodea caliginosa,” in Proceedings of the International Conference Devoted to the 90th Anniversary of the Rostov State University “Ecology and Biology of Soils,“ Rostov-on-Don, 2005 (Rosizdat, Rostov-on-Don, 2005), Vol. 2, pp. 243–246.
B. R. Striganova, O. E. Marfenina, and V. A. Ponomarenko, “Some New Aspects of the Impact of Earthworms on Soil Fungi,” Izv. Akad. Nauk SSSR, Ser. Biol., No. 5, 715–719 (1988).
A. V. Tiunov, “Effect of Earthworm Nisodrilus caliginosus on Nitrification in Soddy-Podzolic Soils,” in Destruction of Organic Matter in the Soil (Vilnius, 1989), pp. 172–176 [in Russian].
J. W. Foster, Chemical Activities of Fungi (Academic, New York, 1949).
S. A. Kharin and A. V. Kurakov, “Mycobiota of Fresh Earthworm Coprolites,” in Proceedings of the International Conference on Environmental Biotechnology, Moscow, Russia, 2006 (Grafikon, Moscow, 2006), pp. 188–195 [in Russian].
J. M. Anderson, “Animal-Microbial Interactions in Soil Biological Processes,” in Straw Decay and Its Effect on Disposal and Utilization: Proceedings of a Symposium on Straw Decay and Workshop on Assessment Techniques, Hatfield, UK, 1979 (Wiley, London, 1979), pp. 311–312.
B. S. Ausmus, N. T. Edwards, and M. Witkamp, “Microbial Immobilization of Carbon, Nitrogen, Phosphorus and Potassium: Implications for Forest Ecosystem Processes,” in The Role of Terrestrial and Aquatic Organisms in Decomposition Processes (Blackwell, Oxford, 1976), pp. 397–416.
Decaëns, A. F. Rangel, N. Asakawa and R. J. Thomas, “Carbon and Nitrogen Dynamics in Ageing Earthworm Cast in Grasslands of the Eastern Plains of Colombia,” Biol. Fertil. Soils 30, 20–28 (1999).
W. Devliegher and W. Verstraete, “Lumbricus terrestris in a Soil Core Experiment: Nutrient-Enrichment Processes (NEP) and Gut-Associated Processes (GAP) and Their Effect on Microbial Biomass and Microbial Activity,” Soil Biol. Biochem. 27, 1573–1580 (1995).
H. L. Drake and M. A. Horn, “As the Worms Turns: the Earthworm Gut as a Transient Habitat for Soil Microbial Biomes,” Annu. Rev. Microbiol. 61, 169–189 (2007).
C. G. Jones, J. H. Lawton, and M. Shachak, “Positive and Negative Effects of Organisms as Physical Ecosystem Engineers,” Ecology 78, 1946–1957 (1997).
G. R. Karsten and H. L. Drake, “Denitrifying Bacteria in the Earthworm Gastrointestinal Tract and in Vivo Emission of Nitrous Oxide (N2O) by Earthworms,” Appl. Environ. Microbiol. 63, 1878–1882 (1997).
P. Lavelle and A. V. Spain, Soil Ecology (Kluwer Academic, London, 2001).
F. Makeschin and G. Benckiser, “Earthworms (Lumbricidae: Oligochaeta): Important Promoters of Soil Development and Soil Fertility,” in Fauna in Soil Ecosystems: Recycling Processes, Nutrient Fluxes, and Agricultural Production, Ed. by G. Benckiser (Dekker, New York, 1997), pp. 173–223.
C. Matthies, A. Griesshammer, M. Schmittroth, and H. L. Drake, “Evidence for Involvement of Gut-Associated Denitrifying Bacteria in Emission of Nitrous Oxide (N2O) by Earthworms Obtained from Garden and Forest Soils,” Appl. Environ. Microbiol. 65, 3599–3604 (1999).
W. B. McGill, H. W. Hunt, R. G. Woodmansee, and J. O. Reuss, “Phoenix, a Model of the Dynamics of Carbon and Nitrogen in Grassland Soils,” in Terrestrial Nitrogen Cycles: Processes, Ecosystem Strategies, and Management Impact, Ecol. Bull. (Stockholm, 1981), Vol. 33, pp. 49–115.
K. Mengel, “Turnover of Organic Nitrogen in Soils and Its Availability to Crops,” Plant Soil 181, 83–96 (1996).
T. B. Parkin and E. C. Berry, “Microbial Nitrogen Transformations in Earthworm Burrows,” Soil Biol. Biochem. 31, 1765–1771 (1999).
T. B. Parkin and E. C. Berry, “Nitrogen Transformations Associated with Earthworm Casts,” Soil Biol. Biochem. 26, 1233–1238 (1994).
S. Scheu, “Microbial Activity and Nutrient Dynamics in Earthworm Casts (Lumbricidae),” Biol. Fertil. Soils 5, 230–234 (1987).
S. Scheu and D. Parkinson, “Changes in Bacterial and Fungal Biomass-C, Bacterial and Fungal Biovolume and Ergosterol Content after Drying, Remoistening, and Incubation of Different Layers of Cool Temperate Forest Soils,” Soil Biol. Biochem. 26, 1515–1525 (1994).
J. Scullion, R. Goodacre, G. Elliott, et al., “Food Quality and Microbial Succession in Ageing Earthworm Casts: Standard Microbial Indices and Metabolic Fingerprinting,” Pedobiologia 47, 888–894 (2003).
A. V. Tiunov and S. Scheu, Microbial Biomass, Biovolume, and Respiration in Lumbricus terrestris L. Cast Material of Different Age, Soil Biol. Biochem. 32, 265–275 (2000).
V. Wolters and R. G. Joergensen, “Microbial Carbon Turnover in Beech Forest Soils Worked by Aporrectodea caliginosa (Savigny) (Oligochaeta: Lumbricidae),” Soil Biol. Biochem. 24, 171–177 (1992).
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Original Russian Text © S.A. Kharin, A.V. Kurakov, 2009, published in Pochvovedenie, 2009, No. 1, pp. 86–92.
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Kharin, S.A., Kurakov, A.V. Transformation of nitrogen compounds and dynamics of microbial biomass in fresh casts of Aporrectodea caliginosa . Eurasian Soil Sc. 42, 75–81 (2009). https://doi.org/10.1134/S1064229309010104
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DOI: https://doi.org/10.1134/S1064229309010104