Abstract
The radioactive petide analogue Semax corresponding to the ACTH(4–10) sequence (Met-Glu-His-Phe-Pro-Gly-Pro) with a specific radioactivity of 56 Ci/mmol labeled with tritium at the C-terminal Pro was prepared. The labeled peptide was used for studying the kinetics of Semax penetration into rat brain and blood after its intranasal administration (50 μg/kg, 20 μl of solution) to nonbred white rats of body mass 200–250 g. It was demonstrated that 0.093% of the total introduced radioactivity per gram can be found in the rat brain 2 min after the administration, 80% of this radioactivity belonged to Semax, and the rest, to its metabolites. The peptide undergoes rapid enzymatic degradation, with the tripeptide Pro-Gly-Pro prevailing in biological samples relative to the total content of Semax and its metabolites.
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Abbreviations
- ACTG:
-
adrenocorticotropic hormone
- CDT:
-
1,1′-carbonyl-di-(1,2,4-triazole)
- MSH:
-
melanocyte-stimulating hormones
- CNS:
-
central nervous system
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Original Russian Text © K.V. Shevchenko, I.Yu. Nagaev, L.Yu. Alfeeva, L.A. Andreeva, A.A. Kamenskii, V.P. Shevchenko, I.A. Grivennikova, N.F. Myasoedov, 2006, puglished in Bioogranicheskaya Khimiya, 2006, Vol. 32, No. 1, pp. 64–70.
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Shevchenko, K.V., Nagaev, I.Y., Alfeeva, L.Y. et al. Kinetics of semax penetration into the brain and blood of rats after its intranasal administration. Russ J Bioorg Chem 32, 57–62 (2006). https://doi.org/10.1134/S1068162006010055
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DOI: https://doi.org/10.1134/S1068162006010055