Abstract
This work considers the structural and mechanical properties of composite materials based on chitosan, as well as montmorillonite micro- and nanoparticles and the possibility of using them for cultivation and targeted delivery of mesenchymal stem and regenerative cells. It has been shown that, upon addition of montmorillonite, the biomaterial acquires stability of structural and mechanical properties under conditions of sterilizational treatment and during manipulations in liquid media in the course of cell cultivation. With the aid of in vitro cultivation with the use of dermal fibroblasts and mesenchymal stem cells of adipose tissue, this material was shown to have a complex of properties providing matrix biocompatibility.
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Abbreviations
- DMSO:
-
dimethylsulfoxide
- ASCs:
-
adipose derived stem cells
- BSA:
-
bovine serum albumin
- Na-MMT:
-
sodium montmorillonite
- MTT:
-
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
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Original Russian Text © P.V. Popryadukhin, I.P. Dobrovolskaya, V.E. Yudin, E.M. Ivan’kova, A.B. Smolyaninov, N.V. Smirnova, 2011, published in Tsitologiya, 2011, Vol. 53, No. 12, pp. 952–958.
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Popryadukhin, P.V., Dobrovolskaya, I.P., Yudin, V.E. et al. Composite materials based on chitosan and montmorillonite: Prospects for use as a matrix for cultivation of stem and regenerative cells. Cell Tiss. Biol. 6, 82–88 (2012). https://doi.org/10.1134/S1990519X12010099
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DOI: https://doi.org/10.1134/S1990519X12010099