Immunotoxic and Genotoxic Effects of Arsenic and Ameliorative Potential of Quercetin and Probiotics in Wistar Rat

Abeer Saeed Alahmari; Khadiga Gamal Eldeen Adham; Ahmad Rashed Alhimaidi

doi:doi:10.11648/j.ajls.20170504.13

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Immunotoxic and Genotoxic Effects of Arsenic and Ameliorative Potential of Quercetin and Probiotics in Wistar Rat

Abeer Saeed Alahmari, Khadiga Gamal Eldeen Adham, Ahmad Rashed Alhimaidi

Published in American Journal of Life Sciences (Volume 5, Issue 4)

Received: 8 July 2017 Accepted: 17 July 2017 Published: 11 August 2017

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Abstract

Arsenic is a significant environmental public health concern. The aim of the present study is to investigate the possible immunotoxic and genotoxic roles of arsenic and the ameliorative effects of quercetin and probiotics as natural antioxidants. This study was performed on male adult Wistar rats divided into six groups: control, NaAsO₂-treated, quercetin-treated, probiotic–treated, NaAsO₂ and quercetin-treated, and NaAsO₂ and probiotics-treated. Blood samples collected from all animals were prepared for some oxidative, immunological and genetic aspects. Administration of arsenic decreased body and spleen weight, reduced serum antioxidant defense parameters and DNA content, increased liver, kidney, and brain weights, plasma malondialdehyde (MDA), myeloperoxidase (MPO), and serum cytokines (IL-1β, IL-6, TNF-α and IFN-γ). Adding quercetin to arsenic was effective in restoring the altered values of cytokines (IL-1β and IL-6), MPO, MDA, catalase (CAT) and reduced glutathione (GSH) induced by arsenic, whereas the presence of probiotics was effective in reducing genotoxicity and improving the changes of cytokines (TNF-α and IFN-γ) and superoxide dismutase (SOD). Quercetin and probiotics are excellent antioxidant therapies, through their ability to suppress reactive oxygen species ROS production, which may contribute to arsenic toxicity.

Published in	American Journal of Life Sciences (Volume 5, Issue 4)
DOI	10.11648/j.ajls.20170504.13
Page(s)	108-115
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Arsenic, Quercetin, Probiotics, Oxidative Stress, Lipid Peroxidation, Inflammation

References

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Abeer Saeed Alahmari, Khadiga Gamal Eldeen Adham, Ahmad Rashed Alhimaidi. (2017). Immunotoxic and Genotoxic Effects of Arsenic and Ameliorative Potential of Quercetin and Probiotics in Wistar Rat. American Journal of Life Sciences, 5(4), 108-115. https://doi.org/10.11648/j.ajls.20170504.13

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ACS Style

Abeer Saeed Alahmari; Khadiga Gamal Eldeen Adham; Ahmad Rashed Alhimaidi. Immunotoxic and Genotoxic Effects of Arsenic and Ameliorative Potential of Quercetin and Probiotics in Wistar Rat. Am. J. Life Sci. 2017, 5(4), 108-115. doi: 10.11648/j.ajls.20170504.13

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AMA Style

Abeer Saeed Alahmari, Khadiga Gamal Eldeen Adham, Ahmad Rashed Alhimaidi. Immunotoxic and Genotoxic Effects of Arsenic and Ameliorative Potential of Quercetin and Probiotics in Wistar Rat. Am J Life Sci. 2017;5(4):108-115. doi: 10.11648/j.ajls.20170504.13

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@article{10.11648/j.ajls.20170504.13,
  author = {Abeer Saeed Alahmari and Khadiga Gamal Eldeen Adham and Ahmad Rashed Alhimaidi},
  title = {Immunotoxic and Genotoxic Effects of Arsenic and Ameliorative Potential of Quercetin and Probiotics in Wistar Rat},
  journal = {American Journal of Life Sciences},
  volume = {5},
  number = {4},
  pages = {108-115},
  doi = {10.11648/j.ajls.20170504.13},
  url = {https://doi.org/10.11648/j.ajls.20170504.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20170504.13},
  abstract = {Arsenic is a significant environmental public health concern. The aim of the present study is to investigate the possible immunotoxic and genotoxic roles of arsenic and the ameliorative effects of quercetin and probiotics as natural antioxidants. This study was performed on male adult Wistar rats divided into six groups: control, NaAsO2-treated, quercetin-treated, probiotic–treated, NaAsO2 and quercetin-treated, and NaAsO2 and probiotics-treated. Blood samples collected from all animals were prepared for some oxidative, immunological and genetic aspects. Administration of arsenic decreased body and spleen weight, reduced serum antioxidant defense parameters and DNA content, increased liver, kidney, and brain weights, plasma malondialdehyde (MDA), myeloperoxidase (MPO), and serum cytokines (IL-1β, IL-6, TNF-α and IFN-γ). Adding quercetin to arsenic was effective in restoring the altered values of cytokines (IL-1β and IL-6), MPO, MDA, catalase (CAT) and reduced glutathione (GSH) induced by arsenic, whereas the presence of probiotics was effective in reducing genotoxicity and improving the changes of cytokines (TNF-α and IFN-γ) and superoxide dismutase (SOD). Quercetin and probiotics are excellent antioxidant therapies, through their ability to suppress reactive oxygen species ROS production, which may contribute to arsenic toxicity.},
 year = {2017}
}

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TY  - JOUR
T1  - Immunotoxic and Genotoxic Effects of Arsenic and Ameliorative Potential of Quercetin and Probiotics in Wistar Rat
AU  - Abeer Saeed Alahmari
AU  - Khadiga Gamal Eldeen Adham
AU  - Ahmad Rashed Alhimaidi
Y1  - 2017/08/11
PY  - 2017
N1  - https://doi.org/10.11648/j.ajls.20170504.13
DO  - 10.11648/j.ajls.20170504.13
T2  - American Journal of Life Sciences
JF  - American Journal of Life Sciences
JO  - American Journal of Life Sciences
SP  - 108
EP  - 115
PB  - Science Publishing Group
SN  - 2328-5737
UR  - https://doi.org/10.11648/j.ajls.20170504.13
AB  - Arsenic is a significant environmental public health concern. The aim of the present study is to investigate the possible immunotoxic and genotoxic roles of arsenic and the ameliorative effects of quercetin and probiotics as natural antioxidants. This study was performed on male adult Wistar rats divided into six groups: control, NaAsO2-treated, quercetin-treated, probiotic–treated, NaAsO2 and quercetin-treated, and NaAsO2 and probiotics-treated. Blood samples collected from all animals were prepared for some oxidative, immunological and genetic aspects. Administration of arsenic decreased body and spleen weight, reduced serum antioxidant defense parameters and DNA content, increased liver, kidney, and brain weights, plasma malondialdehyde (MDA), myeloperoxidase (MPO), and serum cytokines (IL-1β, IL-6, TNF-α and IFN-γ). Adding quercetin to arsenic was effective in restoring the altered values of cytokines (IL-1β and IL-6), MPO, MDA, catalase (CAT) and reduced glutathione (GSH) induced by arsenic, whereas the presence of probiotics was effective in reducing genotoxicity and improving the changes of cytokines (TNF-α and IFN-γ) and superoxide dismutase (SOD). Quercetin and probiotics are excellent antioxidant therapies, through their ability to suppress reactive oxygen species ROS production, which may contribute to arsenic toxicity.
VL  - 5
IS  - 4
ER  -

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Author Information

Abeer Saeed Alahmari

Zoology Department, College of Science, King Saud University, Riyadh, Saudi Arabia; Biology Department, College of Science, King Khalid University, Abha, Saudi Arabia
Khadiga Gamal Eldeen Adham

Zoology Department, College of Science, King Saud University, Riyadh, Saudi Arabia; Zoology Department, College of Science, Alexandria University, Alexandria, Egypt
Ahmad Rashed Alhimaidi

Zoology Department, College of Science, King Saud University, Riyadh, Saudi Arabia

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Abeer Saeed Alahmari, Khadiga Gamal Eldeen Adham, Ahmad Rashed Alhimaidi. (2017). Immunotoxic and Genotoxic Effects of Arsenic and Ameliorative Potential of Quercetin and Probiotics in Wistar Rat. American Journal of Life Sciences, 5(4), 108-115. https://doi.org/10.11648/j.ajls.20170504.13

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ACS Style

Abeer Saeed Alahmari; Khadiga Gamal Eldeen Adham; Ahmad Rashed Alhimaidi. Immunotoxic and Genotoxic Effects of Arsenic and Ameliorative Potential of Quercetin and Probiotics in Wistar Rat. Am. J. Life Sci. 2017, 5(4), 108-115. doi: 10.11648/j.ajls.20170504.13

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AMA Style

Abeer Saeed Alahmari, Khadiga Gamal Eldeen Adham, Ahmad Rashed Alhimaidi. Immunotoxic and Genotoxic Effects of Arsenic and Ameliorative Potential of Quercetin and Probiotics in Wistar Rat. Am J Life Sci. 2017;5(4):108-115. doi: 10.11648/j.ajls.20170504.13

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@article{10.11648/j.ajls.20170504.13,
  author = {Abeer Saeed Alahmari and Khadiga Gamal Eldeen Adham and Ahmad Rashed Alhimaidi},
  title = {Immunotoxic and Genotoxic Effects of Arsenic and Ameliorative Potential of Quercetin and Probiotics in Wistar Rat},
  journal = {American Journal of Life Sciences},
  volume = {5},
  number = {4},
  pages = {108-115},
  doi = {10.11648/j.ajls.20170504.13},
  url = {https://doi.org/10.11648/j.ajls.20170504.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20170504.13},
  abstract = {Arsenic is a significant environmental public health concern. The aim of the present study is to investigate the possible immunotoxic and genotoxic roles of arsenic and the ameliorative effects of quercetin and probiotics as natural antioxidants. This study was performed on male adult Wistar rats divided into six groups: control, NaAsO2-treated, quercetin-treated, probiotic–treated, NaAsO2 and quercetin-treated, and NaAsO2 and probiotics-treated. Blood samples collected from all animals were prepared for some oxidative, immunological and genetic aspects. Administration of arsenic decreased body and spleen weight, reduced serum antioxidant defense parameters and DNA content, increased liver, kidney, and brain weights, plasma malondialdehyde (MDA), myeloperoxidase (MPO), and serum cytokines (IL-1β, IL-6, TNF-α and IFN-γ). Adding quercetin to arsenic was effective in restoring the altered values of cytokines (IL-1β and IL-6), MPO, MDA, catalase (CAT) and reduced glutathione (GSH) induced by arsenic, whereas the presence of probiotics was effective in reducing genotoxicity and improving the changes of cytokines (TNF-α and IFN-γ) and superoxide dismutase (SOD). Quercetin and probiotics are excellent antioxidant therapies, through their ability to suppress reactive oxygen species ROS production, which may contribute to arsenic toxicity.},
 year = {2017}
}

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TY  - JOUR
T1  - Immunotoxic and Genotoxic Effects of Arsenic and Ameliorative Potential of Quercetin and Probiotics in Wistar Rat
AU  - Abeer Saeed Alahmari
AU  - Khadiga Gamal Eldeen Adham
AU  - Ahmad Rashed Alhimaidi
Y1  - 2017/08/11
PY  - 2017
N1  - https://doi.org/10.11648/j.ajls.20170504.13
DO  - 10.11648/j.ajls.20170504.13
T2  - American Journal of Life Sciences
JF  - American Journal of Life Sciences
JO  - American Journal of Life Sciences
SP  - 108
EP  - 115
PB  - Science Publishing Group
SN  - 2328-5737
UR  - https://doi.org/10.11648/j.ajls.20170504.13
AB  - Arsenic is a significant environmental public health concern. The aim of the present study is to investigate the possible immunotoxic and genotoxic roles of arsenic and the ameliorative effects of quercetin and probiotics as natural antioxidants. This study was performed on male adult Wistar rats divided into six groups: control, NaAsO2-treated, quercetin-treated, probiotic–treated, NaAsO2 and quercetin-treated, and NaAsO2 and probiotics-treated. Blood samples collected from all animals were prepared for some oxidative, immunological and genetic aspects. Administration of arsenic decreased body and spleen weight, reduced serum antioxidant defense parameters and DNA content, increased liver, kidney, and brain weights, plasma malondialdehyde (MDA), myeloperoxidase (MPO), and serum cytokines (IL-1β, IL-6, TNF-α and IFN-γ). Adding quercetin to arsenic was effective in restoring the altered values of cytokines (IL-1β and IL-6), MPO, MDA, catalase (CAT) and reduced glutathione (GSH) induced by arsenic, whereas the presence of probiotics was effective in reducing genotoxicity and improving the changes of cytokines (TNF-α and IFN-γ) and superoxide dismutase (SOD). Quercetin and probiotics are excellent antioxidant therapies, through their ability to suppress reactive oxygen species ROS production, which may contribute to arsenic toxicity.
VL  - 5
IS  - 4
ER  -

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