Facile Green Synthesis of Reduced Graphene Oxide and Fabrication of Layer by Layer Self-Assembled rGO@Chitosan@rGO@Folic Acid Nanocomposite for Possible Biosensing Application
The present work reports a simple, cost effective and green method for synthesis of reduced graphene oxide (rGO) using an aqueous extract of Azadirachta indica leaves. Fabrication of self assembly of rGO@chitosan@rGO@folic acid (rGO@CS@rGO@FA) was achieved using a novel approach
of Layer by layer (LbL) technique, in potential application for the detection of cancer. The folic acid was loaded on the LbL by physisorption method. The rGO and LbL self assembled rGO@CS@rGO@FA biosensor was characterized using spectroscopic techniques. The fabricated rGO@CS@rGO composite
had particle size of 477.2 nm. The loaded rGO@CS@rGO@FA LbL biosensor could be attached to the folate receptors present on the cancer cells, which are overexpressed for the folic acid. The internalization of the FA loaded rGO@CS@rGO inside the FRspositive cancer cell was confirmed by confocal
laser microscopy. The cell viability assay was done using the MCF-7 cell line. The LBL biosensor developed could be used as a promising tool for point-of-care detection of cancer in vitro.
Keywords: CANCER; FOLIC ACID; LBL; SENSOR
Document Type: Research Article
Publication date: 01 April 2016
- Bionanoscience attempts to harness various functions of biological macromolecules and integrate them with engineering for technological applications. It is based on a bottom-up approach and encompasses structural biology, biomacromolecular engineering, material science, and engineering, extending the horizon of material science. The journal aims at publication of (i) Letters (ii) Reviews (3) Concepts (4) Rapid communications (5) Research papers (6) Book reviews (7) Conference announcements in the interface between chemistry, physics, biology, material science, and technology. The use of biological macromolecules as sensors, biomaterials, information storage devices, biomolecular arrays, molecular machines is significantly increasing. The traditional disciplines of chemistry, physics, and biology are overlapping and coalescing with nanoscale science and technology. Currently research in this area is scattered in different journals and this journal seeks to bring them under a single umbrella to ensure highest quality peer-reviewed research for rapid dissemination in areas that are in the forefront of science and technology which is witnessing phenomenal and accelerated growth.
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