Elsevier

SLAS Technology

Volume 18, Issue 1, February 2013, Pages 85-98
SLAS Technology

Original Reports
High-Throughput Secondary Screening at the Single-Cell Level

https://doi.org/10.1177/2211068212456978Get rights and content
Under a Creative Commons license
open access

Abstract

We have developed an automated system for drug screening using a single-cell–multiple functional response technology. The approach uses a semiautomated preparatory system, high-speed sample collection, and a unique analytical tool that provides instantaneous results for compound dilutions using 384-well plates. The combination of automation and rapid robotic sampling increases quality control and robustness. High-speed flow cytometry is used to collect single-cell results together with a newly defined analytical tool for extraction of IC50 curves for multiple assays per cell. The principal advantage is the extreme speed of sample collection, with results from a 384-well plate being completed for both collection and data processing in less than 10 min. Using this approach, it is possible to extract detailed drug response information in a highly controlled fashion. The data are based on single-cell results, not populations. With simultaneous assays for different functions, it is possible to gain a more detailed understanding of each drug/compound interaction. Combined with integrated advanced data processing directly from raw data files, the process from sampling to analytical results is highly intuitive. Direct PubMed links allow review of drug structure and comparisons with similar compounds.

Keywords

HTS
high-throughput screening
biology automation
informatics and software
data analysis
screening data analysis
robotics and instrumentation engineering

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