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Gentamicin-Loaded Wound Dressing With Polyvinyl Alcohol/Dextran Hydrogel: Gel Characterization and In Vivo Healing Evaluation

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Abstract

To develop a gentamicin-loaded wound dressing, cross-linked hydrogel films were prepared with polyvinyl alcohol (PVA) and dextran using the freezing–thawing method. Their gel properties such as gel fraction, swelling, water vapor transmission test, morphology, tensile strength, and thermal property were investigated. In vitro protein adsorption test, in vivo wound healing test, and histopathology were performed. Dextran decreased the gel fraction, maximum strength, and thermal stability of hydrogels. However, it increased the swelling ability, water vapor transmission rate, elasticity, porosity, and protein adsorption. The drug gave a little positive effect on the gel properties of hydrogels. The gentamicin-loaded wound dressing composed of 2.5% PVA, 1.13% dextran, and 0.1% drug was more swellable, flexible, and elastic than that with only PVA because of its cross-linking interaction with PVA. In particular, it could provide an adequate level of moisture and build up the exudates on the wound area. From the in vivo wound healing and histological results, this gentamicin-loaded wound dressing enhanced the healing effect more compared to conventional product because of the potential healing effect of gentamicin. Thus, this gentamicin-loaded wound dressing would be used as a potential wound dressing with excellent forming and improved healing effect in wound care.

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Acknowledgments

This research was supported by the Regional R&D Cluster Project designated by the Ministry of Science and Technology & the Ministry of Commerce, Industry, and Energy (2009) and a grant from the Korean Health Technology R&D Project, Ministry for Health, Welfare and Family Affairs, Republic of Korea (A092018).

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Authors and Affiliations

  1. College of Pharmacy, Yeungnam University, 214-1, Dae-Dong, Gyongsan, 712-749, South Korea

    Ma-Ro Hwang, Jong Oh Kim, Jeong Hoon Lee, Yong Il Kim, Jung Ae Kim, Chul Soon Yong & Han-Gon Choi

  2. Dong-A Pharm. Co. Ltd., Yongin-Si, Kyunggi-Do, 449-905, South Korea

    Jeong Hoon Kim, Sun Woo Chang & Sung Gju Jin

  3. School of Textiles, Yeungnam University, 214-1, Dae-Dong, Gyongsan, 712-749, South Korea

    Won Seok Lyoo & Sung Soo Han

  4. College of Oriental Medicine, Daegu Haany University, Gyongsan, 712-715, South Korea

    Sae Kwang Ku

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  1. Ma-Ro Hwang
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Correspondence to Chul Soon Yong or Han-Gon Choi.

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Hwang, MR., Kim, J.O., Lee, J.H. et al. Gentamicin-Loaded Wound Dressing With Polyvinyl Alcohol/Dextran Hydrogel: Gel Characterization and In Vivo Healing Evaluation. AAPS PharmSciTech 11, 1092–1103 (2010). https://doi.org/10.1208/s12249-010-9474-0

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  • DOI: https://doi.org/10.1208/s12249-010-9474-0

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