1996 Volume 44 Issue 8 Pages 1585-1587
Trypsin-catalyzed peptide synthesis has been studied by using p-guanidinophenyl esters of Nα-(tert-butyloxycarbonyl)amino acid and peptide as acyl donor components. The reaction conditions were optimized for organic solvents, pH, and concentration of acceptor. The method was especially useful for the preparation of various peptides containing D-amino acids. The enzymatic hydrolysis of the resulting products was negligible.