Abstract
The agarose gelling system was investigated with the fluorescence polarization method using fluorescent dyes, such as uranine (fluorescein sodium), as an extrinsic probe. The method detected three critical points, c1, c2, and c3, in the concentration change of agarose at room temperatare, corresponding to the concentrations at which the agarose domain occupies the whole solution, the junction zone is made to gel, and the network is presumably completed to hold gel elasticity, respectively. Three transition points, T1, T2, and T3, were also detected in the temperature range studied for these systems. T1 and T3 are well defined transition points of agarose, the gel point and the melting point. T2 is an unknown, new transition point. In order to examine the transition at T2, some other solution properties were determined with the fluorescence polarization method, and T2 was interpreted to be the coil—helix transition temperature of agarose. Thus the fluorescence polarization method provides useful information for the investigation of a polymer gelling system such as agarose.
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Hayashi, A., Kinoshita, K. & Kuwano, M. Studies of the Agarose Gelling System by the Fluorescence Polarization Method. I.. Polym J 9, 219–225 (1977). https://doi.org/10.1295/polymj.9.219
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DOI: https://doi.org/10.1295/polymj.9.219
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