In this paper the working mechanism of Stirling engine is studied from the standpoint of thermoacoustic framework. The work flux measurement is performed in a glass tube equipped with/without a regenerator-heat exchanger assembly. An atmospheric pressure air confined in the tube is periodically perturbed by two speakers at the same frequency (=48Hz) but out of phase. It is experimentally demonstrated that the phasing of two pistons in the Stirling engine (alpha arrangement type) plays the role in creating a steady work flux from the compression piston to the expansion piston, whereas a differentially heated regenerator in the engine operates as a power amplifier for the traveling wave propagating up the temperature gradient.