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A Novel Medium Poly(vinyl acetate-triallyl isocyanurate-divinylbenzene) Coupled with Oligo-β-Cyclodextrin for the Isolation of Puerarin from Pueraria Flavones

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Abstract

Poly(vinyl acetate-triallyl isocyanurate-divinylbenzene) matrix coupled with oligo-β-cyclodextrin was synthesized to separate puerarin. The separation of three isoflavone homologues, puerarin, daidzin and daidzein, was achieved by increasing the proportion of methanol from 40 to 85%. The interaction mechanism between the crosslinkers and puerarin was investigated through molecular dynamics simulation and the mixed-mode character was demonstrated by computational and conformational methods. The research confirms that in order to enhance the recognition, the addition of triallyl isocyanurate is feasible. Meanwhile, an alternative matrix for the separation of puerarin was provided, with the advantages of moderate mechanical rigidity, ease of modification and low cost.

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Acknowledgments

The authors would like to express their appreciation of the financial support obtained from the National Natural Science Foundation of China (Grant Nos.20876011, 20806006 and 20636010), National Basic Research Program of China (973 Program, Grant No.2007CB714304), National High Technology Research and Development Program of China (863 program, Grant No.2007AA100404, 2007AA10Z360).

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Authors and Affiliations

  1. Beijing Key Laboratory of Bioprocess, Beijing University of Chemical Technology, Beijing, 100029, China

    Rong Li, Rui Zhao, Haiyang Zhang, Cong Li, Di Feng, Peiyong Qin & Tianwei Tan

Authors
  1. Rong Li
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  2. Rui Zhao
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  3. Haiyang Zhang
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  4. Cong Li
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  5. Di Feng
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  6. Peiyong Qin
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  7. Tianwei Tan
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Correspondence to Tianwei Tan.

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Li, R., Zhao, R., Zhang, H. et al. A Novel Medium Poly(vinyl acetate-triallyl isocyanurate-divinylbenzene) Coupled with Oligo-β-Cyclodextrin for the Isolation of Puerarin from Pueraria Flavones. Chroma 72, 47–54 (2010). https://doi.org/10.1365/s10337-010-1625-7

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