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Formulations of Biopesticides

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Biopesticides: Use and Delivery

Part of the book series: Methods in Biotechnology ((MIBT,volume 5))

Abstract

A large number of factors can potentially affect the economic feasibility of any given biological control product. These include the impact on the target pest, market size and spectrum of pests affected by the biocontrol agent, vari ability of field performance, costs of production, and a number of technologi cal challenges, including fermentation, formulation, and delivery systems (142). Selection of the appropriate formulations that can improve product sta bility and viability may reduce inconsistency of field performance of many potential biological control agents ((2), 5, 6). It has been indicated that slow progress in research on formulation and delivery systems is a major hurdle to the development of biopesticide products ((1),(7)). This chapter summarizes the efforts and successes toward formulation of biocontrol products for use against diseases (biofungicides), weeds (bioherbicides), and insect pests (bioinsecticides). The discussion emphasizes the use of bacteria, fungi, and viruses as the agents. Information on formulation of other important biocontrol agents, such as nematodes, can be found elsewhere (8).

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References

  1. Auld, B A and Monn, L(1995) Constraints in the development of bioherbicides Weed Technol 9, 638–652

    Google Scholar 

  2. Boyetchko, S M(1996) Impact of soil microorganisms on weed biology and ecology. Phytoprotection 77, 41–56

    Google Scholar 

  3. Jacobsen, B J. and Backman, P. A(1993) Biological and cultural plant disease controls: Alternatives and supplements to chemicals in 1PM systems Plant Dis 77, 311–315

    Google Scholar 

  4. Reichelderfer, K (1984) Factors affecting the economic feasibility of the biological control of weeds, in Proceedings of VI International Symposium on Biological Control of Weeds(Delfosse, E. S, ed), Agnc Can Bull, pp. 135–144

    Google Scholar 

  5. Glass, D. J (1993) Commercialization of soil microbial technologies, in Soil Microbial Ecology Applications in Agricultural and Environmental Management Blaine Metting, F., Jr, ed), Marcel Dekker, New York, pp 595–618

    Google Scholar 

  6. Greaves, M P(1993) Formulation of microbial herbicides to improve perfor-mance in the field, in Proceedings of 8th EWRS Symposium “Quantitative Approaches in Weed and Herbicide Research and Their Practical Application, Braunschweig, Germany, pp. 219–225.

    Google Scholar 

  7. Lumsden, R. D, Lewis, J A, and Fravel, D. R (1995) Formulation and dehveiy of biocontrol agents for use against soilborne plant pathogens, in Biorational Pest Control Agents Formulation and Delivery (Hall, F. R and Barry, J W, eds), ACS Symposium Senes 595, Washington, DC, pp. 166–182.

    Chapter  Google Scholar 

  8. Georgis, R., Dunlop, D B, and Grewal, P S. (1995) Formulation of entomopathogenic nematodes, in Biorational Pest Control Agents Formulation and Delivery(Hall, F R and Barry, J W, eds), ACS Symposium series 595, Washington, DC, pp 197–205

    Google Scholar 

  9. Boyetchko, S. M(1996) Formulating bacteria for biological weed control, in Proceedings of Expert Committee on Weeds, Victoria, Canada, pp 85–87

    Google Scholar 

  10. Powell, K A, and Jutsum, A. R(1993) Technical and commercial aspects of biocontrol products Pesticide Sci 37, 315–321

    Article  Google Scholar 

  11. Boyette, C. D, Quimby, P C, Jr., Caesar, A. J, Birdsall, J L, Connick, W J, Jr., Daigle, D. J, Jackson, M. A, Egley, G. H, and Abbas, H K(1996) Adjuvants, formulations, and spraying systems for improvement of mycoherbicides WeedTechnol 10, 637–644.

    CAS  Google Scholar 

  12. Mclntyre, J L. and Press, L S(1991) Formulation, delivery systems and marketing of biocontrol agents and plant growth promoting rhizobactena (PGPR), inThe Rhizosphere and Plant Growth (Keister, D L. and Cregan, P B, eds), Beltsville Symposia in Agricultural Research, Beltsville, MD, pp 289–295.

    Google Scholar 

  13. Bailey, K L., Boyetchko, S M., Mortensen, K, and Wolf, T. M. (1997) Biological control of weeds using plant pathogens, in Proceedings of Soils & Crops Workshop, Saskatoon, Saskatchewan, Canada, pp. 205–210

    Google Scholar 

  14. Paau, A. S(1988) Formulations useful in applying beneficial microorganisms to seed. TibTech 6, 276–279

    Google Scholar 

  15. van Elsas, J. D. and Heynen, C. E. (1990) Methods for the introduction of bacteria into soil: A review. Biol Fertil Soils 10, 127–133

    Google Scholar 

  16. Harman, G E. (1991) Production and delivery systems for biocontrol agents, in New Approaches in Biological Control of Soil-Borne Diseases (Schoenbeck, F, ed), Copenhagen, Denmark, pp. 201–205

    Google Scholar 

  17. Fages, J (1992) An industrial view of Azospmllum inoculants formulation and application technology Symbiosis 13, 15–26

    Google Scholar 

  18. Bryant, J E. (1994) Commercial production and formulation of Bacillus thuringiensis. Agnc Ecosys Environ 49, 31–35

    Article  Google Scholar 

  19. Digat, B. (1989) Strategies for seed bactenzation. Acta Hortic. 253, 121–130

    Google Scholar 

  20. Jha, P. K., Nair, S., and Babu, S. (1993) Encapsulation of seeds of Sesbama sesban with polyacrylamide and alginate gel entrapped rhizobia leads to effective symbiotic nitrogen fixation Ind J Expt Biol. 31, 161–167.

    CAS  Google Scholar 

  21. Digat, B. (1991) A new encapsulation technology for bacterial inoculants and seed bactenzation, in Plant Growth-Promoting Rhizobacteria. Progress and Prospects International Workshop on Plant Growth-Promoting Rhizobacteria Interlaken, Switzerland, Bulletin SROP, No 14, pp 383–391

    Google Scholar 

  22. Stafford, C J(1995) Production and formulation of Bt, in Biopesticides Opportunities for Australian Industry (Monsour, C. J, Reid, S., and Teakle, A E., eds), Proceedings of the 1st Brisbane Symposium, University of Queensland, pp 78–83

    Google Scholar 

  23. Johnson, D R., Wyse, D L., and Jones, K J. (1996) Controlling weeds with phytopathogenic bacteria Weed Technol 10, 621–624.

    Google Scholar 

  24. Zidack, N K, Backman, P A, and Shaw, J J(1992) Promotion of bacterial infection of leaves by an organosilicone surfactant’ implications for biological weed control Biol Control 2, 111–117

    Article  Google Scholar 

  25. Imaizumi, S, Nishino, T, Miyabe, K., Fujimori, T, and Yamada, M(1997) Biological control of annual bluegrass (Poa annua L.) with a Japanese isolate of Xanthomonas campestris pv. poae (JT-P482) Biol Control 8, 7–14

    Article  Google Scholar 

  26. Zidack, N K. and Backman, P A(1996) Biological control of kudzu (Puerana lobata) with the plant pathogen Pseudomonas syringae pv. phaseohcola Weed Sci 44, 645–649

    CAS  Google Scholar 

  27. Cook, R. J. (1993) Making greater use of introduced microorganisms for biological control of plant pathogens Annu Rev Phytopathol 31, 53–80

    Article  PubMed  CAS  Google Scholar 

  28. Elad, Y and Chet, I (1995) Practical approaches for biocontrol implementation, in Novel Approaches to Integrated Pest Management (Reuveni, R, ed), Lewis, London, pp. 323–338

    Google Scholar 

  29. Shouan, Z Weimin, X., Zhinong, Y, and Ruhong, M (1996) Research and commercialization of yield-increasing bacteria (YIB) in China, in Advances of Biological Control of Plant Diseases (Wenhua, T, Cook, R J, and Rovira, A, eds), Proceedings of the International Workshop on Biological Control of Plant Diseases, Beijing, China, pp 47–53

    Google Scholar 

  30. Kerr, A. (1980) Biological control of crown gall through production of agrocin 84 Plant Dis 64, 25–30

    Google Scholar 

  31. Cannon, R. J. C (1993) Prospects and progress for Bacillus thurmgiensis-based pesticides Pestic. Sci 37, 331–335

    Article  Google Scholar 

  32. Shieh, T R (1995) Biopesticide formulations and their applications, in Proceedings of American Chemical Society (Ragsdale, N. N, Kearney, P C, and Plimmer, J R., eds.), Eighth International Congress of Pesticide Chemistry Options 2000, Washington, DC, pp 104–114

    Google Scholar 

  33. Gaertner, F. H., Quick, T C, and Thompson, M. A. (1993) CellCap: an encapsulation system for insecticidal biotoxin proteins, in Advanced Engineered Pesticides (Kim, L., ed.), Marcel Decker, New York, pp. 73–83.

    Google Scholar 

  34. Panetta, J. D (1993) Engineered microbes: the CellCap system, in Advanced Engineered Pesticides (Kim, L, ed.), Marcel Decker, New York, pp. 379–382

    Google Scholar 

  35. Burnett, H C, Tucker, D P H., and Ridings, W. H. (1974) Phytophthora root and stem rot of milkweed vine. Plant Dis Rep 58, 355–357.

    Google Scholar 

  36. TeBeest, D. O and Templeton, G. E. (1985) Mycoherbicide: progress in the biological control of weeds. Plant Dis 69, 6–10.

    Google Scholar 

  37. Boyette, C. D. (1994) Unrefined corn oil improves the mycoherbicidal activity of Colletotnchum truncation for hemp sesbania (Sesbania exaltata) control Weed Technol 8, 526–529

    CAS  Google Scholar 

  38. Connick, W. J., Jr, Lewis, J A., and Quimby, P C, Jr. (1990) Foimulation of biocontrol agents for use in plant pathology. UCLA Symposium in Molecular and Cell Biology, pp. 345–372.

    Google Scholar 

  39. Daigle, D J, Connick, W. J, Jr., Quimby, P C, Jr., Evans, J., Trask-Morrell, B, and Fulgham, F. E (1990) Invert emulsionscarrier and water source for the mycoherbicide Alternaria cassiae. Weed Technol 4, 327–331.

    CAS  Google Scholar 

  40. Boyette, C D, Quimby, P. C, Jr., Bryson, C. T., Egley, G. H, and Fulgham, F. E (1993) Biological control of hemp sesbania (Sesbania exaltata) under field conditions with Colletotnchum truncatum formulated in an invert emulsion WeedSci 41, 497–500

    Google Scholar 

  41. Walker, H L. and Boyette, C. D. (1985) Biocontrol of sicklepod (Cassia obtusifoha) in soybeans (Glycine max) with Alternaria cassiae. Weed Set 33, 212–215.

    Google Scholar 

  42. Amsellem, Z, Sharon, A, Fressel, J., and Quimby, P. C, Jr(1990) Complete abolition of high inoculum threshold of two mycoherbicides (Alternaria cassiae and A crassa) when applied in invert emulsion Phytopathology 80, 925–929

    Article  Google Scholar 

  43. Daigle, D. J. and Connick, W J, Jr(1990) Formulation and application technology for microbial weed control, in Microbes and Microbial Products as Herbicides (Hoagland, R. E, ed.), ACS Symposium series 439, American Chemical Society, Washington, DC, pp 288–304.

    Chapter  Google Scholar 

  44. Connick, W. J, Jr, Daigle, D J, and Quimby, P. C, Jr(1991) An improved invert emulsion with high water retention for mycoherbicide delivery. Weed Technol 5, 442–444

    CAS  Google Scholar 

  45. Auld, B. A (1993) Vegetable oil suspension emulsions reduce dew dependence of a mycoherbicide. Crop Prot. 12, 477–479

    Article  Google Scholar 

  46. Daigle, D. J and Cotty, P. J (1992) Production of comdia of Alternaria cassiae with alginate pellets Biol Control 2, 278–281

    Article  Google Scholar 

  47. Connick, W. J., Jr., Boyette, C. D., and McAlpine, J R (1991) Formulation of mycoherbicides using a pasta-like process. Biol Control 1, 281–287.

    Article  Google Scholar 

  48. Connick, W J., Jr., Daigle, D. J., Boyette, C. D., Williams, K. S., and Vinyard, B. (1996) Water activity and other factors that affect the viability of Colletotnchum truncatum comdia in wheat flour-kaolin granules (’ pesta’). Biocontrol Sci Technol 6, 277–284

    Article  Google Scholar 

  49. Boyette, C. D., Templelton, G. E., and Oliver, L. R. (1985) Texas gourd (Cucurbita texana) control with Fusanum solam f.sp. cucurbitae. Weed Sci 32, 649–654

    Google Scholar 

  50. Belanger, R. R. and Benyagoub, M(1997) Challenges and prospects for integrated control of powdery mildews in the greenhouse. Can J. Plant Pathol 19, 310–314

    Article  Google Scholar 

  51. Urquhart, E. J and Punja, Z. K(1997) Epiphytic growth and survival of Tilletwpsis pallescens, a potential biological control agent of Sphaerotheca fuhginea, on cucumber leaves. Can J Bot. 75, 892–901.

    Google Scholar 

  52. Punja, Z. K(1997) Comparative efficacy of bacteria, fungi, and yeasts as biological control agents for diseases of vegetable crops Can J Plant Pathol 19, 315–323

    Article  Google Scholar 

  53. Lacey, L A and Goettel, M S. (1995) Current developments in microbial control of insect pests and prospects for the early 21 st century Entomophaga 40, 3–27

    Article  Google Scholar 

  54. Bateman, R (1997) The development of a mycomsecticide for the control of locusts and grasshoppers. Outlook Agncult 26, 13–18.

    Google Scholar 

  55. Feng, M G, Poprawski, T J, and Khachatounans, G G (1994) Production, formulation and application of the entomopathogenic fungus Beauvena bassiana for insect control: current status. Biocontrol Sci Technol 4, 3–34

    Article  Google Scholar 

  56. Goettel, M S, Johnson, D. L, and Inglis, G. D. (1995) The role of fungi in the control of grasshoppers. Can J Bot 73, 571–575

    Article  Google Scholar 

  57. Inghs, G D, Johnson, D L., and Goettel, M. S (1996) Effect of bait substrate and formulation on infection of grasshopper nymphs by Beauvena bassiana Biocontrol Sci Technol 6, 35–50

    Google Scholar 

  58. Auld, B. A(1992) Mass production, formulation and application of fungi as biocontrol agents, in Biological Control of Locusts and Grasshoppers CAB International, Walhngford, UK, pp 219–229

    Google Scholar 

  59. Caudwell, R. W. and Gatehouse, A G (1996) Laboratory and field trial of bait formulations of the fungal pathogen, Metarhizium flavovinde, against a tropical grasshopper and locust Biocontrol Sci Technol 6, 561–567

    Article  Google Scholar 

  60. Daoust, R A. and Pereira, R M(1986) Stability of the entomopathogenic fungi Beauvena bassiana and Metarhizium anisophae on clbeetle-attracting tubers and cowpea foliage in Brazil. Environ Entomol 15, 1237–1243

    Google Scholar 

  61. Moore, D, Bridge, P D, Higgins, P M, Bateman, R P, and Prior, C (1993) Ultra-violet radiation damage to Metarhizium flavoviride conidia and the protection given by vegetable and mineral oils and chemical sunscreens Ann Appl Biol 122, 605–615.

    Article  CAS  Google Scholar 

  62. Bateman, R P, Carey, M, Moore, D., and Prior, C (1993) The enhanced mfectivity of Metarhizium flavovinde in oil formulations to desert locusts at low humidities. Ann Appl Biol 122, 145–152

    Article  Google Scholar 

  63. Moore, D., Bateman, R. P, Carey, M., and Prior, C (1995) Long-term storage of Metarhizium flavoviride conidia in oil formulations for the control of locusts and grasshoppers. Biocontrol Sci Technol. 5, 193–199

    Article  Google Scholar 

  64. McGuire, M R. and Shasha, B. S. (1992) Adherent starch granules for encapsulation of insect control agents J Econ Entomol 85, 1425–1433

    Google Scholar 

  65. Pereira, R M. and Roberts, D W (1991) Alginate and cornstarch mycelial formulations of entomopathogenic fungi, Beauvena bassiana and Metarhizium anisophae. J Econ Entomol 84, 1657–1661

    Google Scholar 

  66. Caudwell, R. W. and Gatehouse, A G (1996) Formulation of grasshopper and locust entomopathogens in baits using starch extrusion technology Crop Prot 15, 33–37.

    Article  Google Scholar 

  67. Cory, J. S. and Bishop, D H. L (1995) Use of baculoviruses as biological insecticides, in Methods in Molecular Biology, vol 39: Baculovirus Expression Protocols (Richardson, C D., ed), Humana, Totowa, NJ, pp. 277–294

    Chapter  Google Scholar 

  68. Shapiro, M. (1995) Radiation protection and activity enhancement of viruses, in Biorational Pest Control Agents. Formulation and Delivery (Hall, F R. and Barry, J. W., eds.), ACS Symposium Series 595, Washington, DC, pp 153–164.

    Google Scholar 

  69. Shapiro, M. (1992) Use of optical bnghteners as radiation protectants for gypsy moth (Lepidoptera.Lymantriidae) nuclear polyhedrosis virus J Econ Entomol 85, 1682–1686

    CAS  Google Scholar 

  70. Shapiro, M. and Robertson, J. L (1992) Enhancement of gypsy moth (Lepidoptera: Lymantnidae) baculovirus activity by optical brighteners. J Econ Entomol 85, 1120–1124

    CAS  Google Scholar 

  71. Dougherty, E. M, Guthrie, K., and Shapiro, M. (1995) In vitro effects of fluorescent brightener on the efficacy of occlusion body dissolution and polyhedralderived virions Biol Control 5, 383–388

    Article  Google Scholar 

  72. Erlandson, M A and Moore, K. C. (1994) Enhancement of bertha armyworm baculovirus activity by an optical brightener compound, in Proceedings of 42nd Annual meeting of the Entomological Society of Alberta, Canmore, Alberta, Canada, p. 11

    Google Scholar 

  73. Cook, R J and Baker, K R (1983) The Nature and Practice ojBiological Control of Plant Pathogens American Phytopathological Society, St Paul, MN, 539 pp.

    Google Scholar 

  74. Kommedahl, T. and Wmdels, C E. (1981) Introduction of microbial antagonists to specific courts of infection seeds, seedlings, and wounds, in Bwcontrol in Crop Production (Papavizas, G C, ed.), BARC Symposium 5 Allanheld and Osmun, Totowa, NJ, pp 227–248

    Google Scholar 

  75. Lewis, J A (1991) Formulation and delivery systems of biocontrol agents with emphasis on fungi, in The Rhizosphere and Plant Growth (Keister, D L and Cregan, P B, eds), Kluwer, Dordrecht, The Netherlands, pp 279–287

    Google Scholar 

  76. Thomashow, L S and Weller, D. M(1990) Application of fluorescent pseudomonads to control root diseases of wheat and some mechanisms of disease suppression, in Biological Control of Soil-Borne Plant Pathogens (Hornby, D, ed.), Redwood, Melksham Wiltshire, UK, pp 109–122

    Google Scholar 

  77. Gindrat, D. (1979) Biocontrol of plant diseases by inoculation of fresh wounds, seeds and soil with antagonists, in Soil-Borne Plant Pathogens (Schippers, B. and Grams, W., eds), Academic, New York, pp 537–551.

    Google Scholar 

  78. Elliott, L F and Lynch, J. M (1985) Plant growth-inhibiting pseudomonads colonizing winter wheat (Triticum aestivum L) roots Plant Soil 84, 57–65.

    Article  Google Scholar 

  79. Frednckson, J. K, Elliott, L F, and Engibous, J C (1987) Crop residues as substrates for host-specific inhibitory pseudomonads. Soil Biol Biochem 19, 127–134

    Article  Google Scholar 

  80. Stroo, H F, Elliott, L. F., and Papendick, R I (1988) Growth, survival and toxin production of root-inhibitory pseudomonads on crop residues. Sod Biol Biochem 20, 201–207

    Article  Google Scholar 

  81. Funk, L M, He, D N, Pedersen, E A., and Reddy, M. S (1997) Optimization of product delivery for a microbial inoculant, Burkholderia cepacia, for commercial use in the forest industry (Abstr). Can J Plant Pathol 19, 108

    Google Scholar 

  82. Rishbeth, J (1975) Stump inoculation: A biological control of Fomes annosus, in Biology and Control of Soil-Borne Plant Pathogens (Bruehl, G W, ed), American Phytopathological Society, St. Paul, MN, pp. 158–162

    Google Scholar 

  83. Harvey, L T. (1991) Guide to Agricultural Spray Adjuvants Usedin the United States Thompson Publications, Fresno, CA

    Google Scholar 

  84. Steinke, W. E. and Akesson, N. B (1993) Atomization of biopesticide formulations, in Pesticide Formulations and Application Systems, Volume 12, ASM STP 1146 (Devisetty, B N., Chasm, D. G., and Berger, P D, eds.), American Society for Testing and Materials, Philadelphia, pp. 257–271

    Google Scholar 

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Boyetchko, S., Pedersen, E., Punja, Z., Reddy, M. (1999). Formulations of Biopesticides. In: Hall, F.R., Menn, J.J. (eds) Biopesticides: Use and Delivery. Methods in Biotechnology, vol 5. Humana Press. https://doi.org/10.1385/0-89603-515-8:487

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  • DOI: https://doi.org/10.1385/0-89603-515-8:487

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-515-7

  • Online ISBN: 978-1-59259-483-2

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