Abstract
Organosolv (ethanol/water and acetosolv) pulps were treated with Humicola grisea var. thermoidea and compared with Cartazyme HS xylanase-treated pulp. The ethanol/water pulps treated with H. grisea had the same viscosity as unbleached pulps (8 cP). Ethanol/water pulps treated with Cartazyme had higher viscosity than H. grisea-treated pulps (12 cP). Acetosolv pulps treated with H. grisea and Cartazyme presented a reduction in viscosity; however, the pulps treated with H. grisea had a lower reduction in viscosity than Cartazyme-treated pulps. Ethanol/water pulps treated with H. grisea had a 23% reduction in kappa number in 4 and 8 h of treatment, compared with the unbleached pulps. Cartazyme-treated pulps had a kappa number similar to that of the control pulps for 4 h of treatment. Extending the treatment time to 12 h resulted in a reduction of 33%. The acetosolv pulp treated with H. grisea had a kappa number reduced to 23% in 4 h. Cartazyme treatment resulted in a reduction of 55 and 44% in kappa number for 4 and 8 h of treatment, respectively, when compared with control pulp. Extending the treatment time to 12 h decreased the kappa number 72%. Fourier transform infrared spectra and principal component analysis showed differences among unbleached, H. grisea-treated, and Cartazyme-treated pulps.
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Salles, B. C., Medeiros, R. G., Báo, S. N., Silva, F. G., and Filho, E. X. F. (2005), Process Biochem. 40, 343–349.
Lima, U. A., Aquarone, E., Borzani, W., and Schmidell, W. in Biotecnologia Industrial: Processos Fermentativos e Enzimáticos, vol. 3, Edgard Blücher, São Paulo, Brazil, 1–593.
Viikari, L., Kantelinen, A., Sundquist, J., and Linko, M. (1994), FEMS Microbiol. 13, 335–350.
Techapum, C., Poosoram, N., Watanabe, M., and Sasaki, K. (2003), Process Biochem. 38, 1327–1340.
Garg, A. P., Roberts, J. C., and McCarthy, A. J. (1998), Enzyme Microb. Technol. 22, 594–598.
Jurasek, L. and Paice, M. G. (1987), Chem. Technol. 16, 360–365.
Almeida, E. M., Polizeli, M. L., Jorge, J. A., and Terenzi, H. F. (1995), FEMS Microbiol. Lett. 130, 171–176.
Faria, F. P., Czifersky, A., Nevalainen, H., Azevedo, O., Gibbs, M., Berquist, P. L., and Teo, V. S. J. (2002), Appl. Biotechnol. Biotec. 98–100, 78–83.
Benar, P. (1992), MS thesis, UNICAMP/Instituto de Química, Campinas-SP, Brazil.
Gonçalves, A. R. and Ruzene, S. D. (2003), Appl. Biochem. Biotechnol. 105–108, 769–774.
Bailey, M. J., Biely, P., and Pourtanen, K. (1992), J. Biotechnol. 23, 257–270.
TAPPI. (1985), TAPPI Standard Methods, T. 236 cm-85.
TAPPI. (1992), TAPPI Standard Methods, T. 230 om-82.
Rocha, G. J. M. (2000), PhD thesis, São Carlos/Universidade de São Paulo, Brazil.
Faix, O., Böttcher, J. H., and Berlelt, E. (1992), Using FTIR spectroscopy and FTIR microscopy for the examination of wood and wood tissue, in 8th International Conference in Fourier Transform Spectroscopy Proceedings SPIE 1575, Heise, M., Korte, E. H., and Siesler, H. W., eds., The International Society for Optical Engineering, San Diego, CA, pp. 428–430.
Scarminio, I. S. and Bruns, R. E. (1989), Trends Anal. Chem. 8, 326–327.
Garg, A. P., Roberts, J. C., and McCarthy, A. J. (1998), Enzyme Microb. Technol. 22, 594–598.
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Moriya, R.Y., Gonçalves, A.R. & Faria, F.P. Enzymatic bleaching of organosolv sugarcane bagasse pulps with recombinant xylanase of the fungus Humicola grisea and with commercial cartazyme HS xylanase. Appl Biochem Biotechnol 121, 195–203 (2005). https://doi.org/10.1385/ABAB:121:1-3:0195
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DOI: https://doi.org/10.1385/ABAB:121:1-3:0195