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Metabolic engineering of Saccharomyces cerevisiae for efficient production of pure l−(+)−lactic acid

  • Session 5 Microbial Catalysis and Metabolic Engineering
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Abstract

We developed a metabolically engineered Saccharomyces cerevisiae, which produces optically pure l-lactic acid efficiently using cane juice-based medium. In this recombinant, the coding region of pyruvate decarboxylase (PDC)1 was completely deleted, and six copies of the bovine l-lactate dehydrogenase (l-LDH) genes were introduced on the genome under the control of the PDC1 promoter. To confirm optically pure lactate production in lowcost medium, cane juice-based medium was used in fermentation with neutralizing conditions. l-lactate production reached 122 g/L, with 61% of sugar being transformed into l-lactate finally. The optical purity of this l-lactate, that affects the physical characteristics of poly-l-lactic acid, was extremely high, 99.9% or over.

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Authors and Affiliations

  1. Biotechnology Laboratory, Toyota Central R&D Labs Inc., 480-1192, Aichi, Japan

    Nobuhiro Ishida, Kenro Tokuhiro, Eiji Nagamori & Haruo Takahashi

  2. Toyota Biotechnology & Afforestation Laboratory, Toyota Motor Co., 470-0201, Aichi, Japan

    Satoshi Saitoh & Toru Ohnishi

  3. Department of Biotechnology, The University of Tokyo, Bunkyo-ku, 113-8657, Tokyo, Japan

    Katsuhiko Kitamoto

Authors
  1. Nobuhiro Ishida
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  2. Satoshi Saitoh
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  3. Toru Ohnishi
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  4. Kenro Tokuhiro
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  5. Eiji Nagamori
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  6. Katsuhiko Kitamoto
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  7. Haruo Takahashi
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Correspondence to Nobuhiro Ishida.

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These two authors contributed equally to this work.

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Ishida, N., Saitoh, S., Ohnishi, T. et al. Metabolic engineering of Saccharomyces cerevisiae for efficient production of pure l−(+)−lactic acid. Appl Biochem Biotechnol 131, 795–807 (2006). https://doi.org/10.1385/ABAB:131:1:795

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  • DOI: https://doi.org/10.1385/ABAB:131:1:795

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