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Modeling the enzymatic hydrolysis of dilute-acid pretreated Douglas Fir

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Abstract

Glucose yield from the enzymatic hydrolysis of cellulose was investigated as a function of cellulase enzyme loading (7–36 filter paper units [FPU]/g cellulose) and solids concentration (7–18% total solids) for up to 72 h on dilute sulfuric-acid pretreated Douglas Fir. The saccharification was performed on whole hydrolysate with no separation or washing of the solids. Enzyme loading had a significant effect on glucose yield; solids concentration had a much smaller effect even at higher glucose concentrations. The data were used to generate an empirical model for glucose yield, and to fit parameters of a cellulose hydrolysis kinetic model. Both models could be used for economic evaluation of a separate hydrolysis and fermentation process.

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Authors and Affiliations

  1. National Renewable Energy Laboratory, 1617 Cole Blvd., 80401-3393, Golden, CO

    Daniel J. Schell, Mark F. Ruth & Melvin P. Tucker

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  1. Daniel J. Schell
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  2. Mark F. Ruth
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  3. Melvin P. Tucker
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Correspondence to Daniel J. Schell.

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Schell, D.J., Ruth, M.F. & Tucker, M.P. Modeling the enzymatic hydrolysis of dilute-acid pretreated Douglas Fir. Appl Biochem Biotechnol 77, 67–81 (1999). https://doi.org/10.1385/ABAB:77:1-3:67

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  • DOI: https://doi.org/10.1385/ABAB:77:1-3:67

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