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SO2-catalyzed steam explosion of corn fiber for ethanol production

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Abstract

Corn fiber, a by-product of the corn wet-milling industry, represents a renewable resource that is readily available in significant quantities and could potentially serve as a low-cost feedstock for the production of fuel-grade alcohol. In this study, we used a batch reactor to steam explode corn fiber at various degrees of severity to evaluate the potential of using this feedstock in the bioconversion process. The results indicated that maximum sugar yields (soluble and following enzymatic hydrolysis) were recovered from corn fiber that was pretreated at 190°C for 5 min with 6% SO2. Sequential SO2-catalyzed steam explosion and enzymatic hydrolysis resulted in very high conversion (81%) of all polysaccharides in the corn fiber to monomeric sugars. Subsequently, Saccharomyces cerevisiae was able to convert the resultant corn fiber hydrolysates to ethanol very efficiently, yielding 90–96% of theoretical conversion during the fermentation process.

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References

  1. Grohmann, K., Torget, R., and Himmel, M. (1985), Biotechnol. Bioeng. Symp. 15, 59–80.

    Google Scholar 

  2. Grohmann, K. and Bothast, R. J. (1996), Process Biochem. 32, 405–415.

    Article  Google Scholar 

  3. Excoffier, G., Toussaint, B., and Vignon, M. R. (1991), Biotechnol. Bioeng. 38, 1308–1317.

    Article  CAS  Google Scholar 

  4. Gregg, D. J., Boussaid, A., and Saddler, J. N. (1998), Bioresour. Technol. 63, 7–12.

    Article  CAS  Google Scholar 

  5. O’Connor, D. (2000), Liquid Fuels from Biomass: North American Impact on Non-technical Barriers on Implementation, Report IEA Bioenergy Task 27, Delta, Canada, pp. 34–46.

  6. Anderson, R. A. and Watson, S. A. (1982), in Handbook of Processing and Utilization in Agriculture, vol. 2, Wolff, I. A. ed., CRC, Boca Raton, FL, pp. 31–61.

    Google Scholar 

  7. Osborn, D. and Chen, L. F. (1984), Starch/Stärke. 36, 393–395.

    Article  CAS  Google Scholar 

  8. Beall, D. S., Ingram, L. O., Ben-Bassat, A., Doran, J. B., Fowler, D. E., Hall, R. G., and Wood, B. E. (1992), Biotechnol. Lett. 14, 857–862.

    Article  CAS  Google Scholar 

  9. Moniruzzaman, M., Dale, B. E., Hespell, R. B., and Bothast, R. J. (1997), Appl. Biochem. Biotechnol. 67, 113–126.

    CAS  Google Scholar 

  10. Clark, T. A. and Mackie, K. L. (1987), J. Wood Chem. Technol. 7, 373–403.

    CAS  Google Scholar 

  11. Schwald, W., Smaridge, T., Chan, M., Breuil, C., and Saddler, J. N. (1987), in Enzyme Systems for Lignocellulose Degradation, Coughlan, M. P. ed., Elsevier, NY, pp. 231–242.

    Google Scholar 

  12. Clark, T. A., Mackie, K. L., Dare, P. H., and McDonald, A. G. (1989), J. Wood Chem. Technol. 9, 135–166.

    CAS  Google Scholar 

  13. Carrasco, J. E., Ma, C., Saiz, A., Navarro, P., Soriano, P., Saez, F., and Martinez, J. M. (1994), Appl. Biochem. Biotechnol. 45,46, 23–34.

    Google Scholar 

  14. Boussaid, A., Esteghlalian, A. R., Gregg, D. J., Lee, K. H., and Saddler, J. N. (2000), Appl. Biochem. Biotechnol. 84–86, 693–705.

    Article  Google Scholar 

  15. Brownell, H. H., Yu, E. K. C., and Saddler, J. N. (1986), Biotechnol. Bioeng. 27, 792–801.

    Article  Google Scholar 

  16. Mackie, K. L., Brownell, H. H., West, K. L., and Saddler, J. N. (1985), J. Wood Chem. Technol. 5, 405–425.

    CAS  Google Scholar 

  17. Chum, H. L., Johnson, D. K., Black, S., Baker, J., Grohmann, K., Sarkanen, K. V., Wallace, K., and Schroeder, H. A. (1988), Biotechnol. Bioeng. 31, 643–649.

    Article  CAS  Google Scholar 

  18. Boussaid, A. and Saddler, J. N. (1999), Enzyme Microb. Technol. 24, 138–143.

    Article  CAS  Google Scholar 

  19. Boussaid, A., Robinson, J., Cai, Y. J., Gregg, D. J., and Saddler, J. N. (1999), Biotechnol. Bioeng. 64, 284–289.

    Article  CAS  Google Scholar 

  20. Shevchenko, S. M., Beatson, R. P., and Saddler, J. N. (1999), Appl. Biochem. Biotechnol. 77–79, 867–876.

    Article  Google Scholar 

  21. Michalowicz, G., Toussaint, B., and Vignon, M. R. (1991), Holzforschung 45, 175–179.

    CAS  Google Scholar 

  22. Overend, R. P. and Chornet, E. (1987), Phil. Trans. R. Soc. Lond. 321, 523–536.

    Article  CAS  Google Scholar 

  23. Brownell, H. H., Schwald, W., Smaridge, T., and Saddler, J. N. (1988), in Proceedings of the IEA Workshop on Bioconversion of Lignocellulosics, Saddler, J. N., Stevens, D., and Hages, D., eds., Forintek Canada, Ottawa, Ontario, pp. A-1.

  24. TAPPI, Technical Association of the Pulp and Paper Industry (1998), TAPPI Standard Methods, T-222 om-98, TAPPI Press, Atlanta, GA.

    Google Scholar 

  25. Ghose, T. K. (1987), Pure Appl. Chem. 59, 257–268.

    CAS  Google Scholar 

  26. Olsson, L., Hahn-Hägerdal, B., and Zacchi, G. (1995), Biotechnol. Bioeng. 45, 356–365.

    Article  CAS  Google Scholar 

  27. Whistler, R. L. (1993), in Industrial Gums: Polysaccharides and Their Derivatives, 3rd ed., Whistler, R. L. and BeMiller, J. N., eds., Academic, San Diego, CA, pp. 294–308.

    Google Scholar 

  28. BeMiller, J. N. (1967), Adv. Carbohydr. Chem., 22, 25–107.

    CAS  Google Scholar 

  29. Sjolander, N. O., Langlykke, A. F., and Peterson, W. F. (1938), Ind. Eng. Chem. 30, 1251–1255.

    Article  CAS  Google Scholar 

  30. Dien, B. S., Iten, L. B., and Bothast, R. J. (1999), J. Ind. Microb. Biotechnol. 22, 575–581.

    Article  CAS  Google Scholar 

  31. Parajó, J. C., Dominguez, H., and Domínquez, J. M. (1997), Enzyme Microb. Technol. 21, 18–24.

    Article  Google Scholar 

  32. Larsson, S., Reimann, A., Nilvebrant, N. O. and Jönsson, L. J. (1999), Appl. Biochem. Biotechnol. 77–79, 91–103.

    Article  Google Scholar 

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Correspondence to Shawn D. Mansfield.

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Bura, R., Mansfield, S.D., Saddler, J.N. et al. SO2-catalyzed steam explosion of corn fiber for ethanol production. Appl Biochem Biotechnol 98, 59–72 (2002). https://doi.org/10.1385/ABAB:98-100:1-9:59

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