Abstract
The technical viability was investigated for the conversion of Pinus pinaster stumps to value-added products via a chain of pretreatments and simultaneous saccharification and fermentation (SSF) steps to obtain bioethanol. Sequential steam explosion (SE), organosolv (OS) pretreatment and soda-anthraquinone pulping (Na/AQ) were performed resulting in an unbleached pulp (UBP), which was successfully converted via SSF to bioethanol with concentrations up to 79 g l−1, which corresponds to a conversion yield of 97% and productivity of 1.09 g l−1 h−1 at 15% total solids. Accordingly, delignification steps by oxygen bleaching are not necessary for bioethanol production. Different industrial softwood (SW) pulps were also tested as reference materials. Total lignin contents up to 4.5% in SW-based pulps had no adverse effects on SSF efficiency.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: This work has been framed under the MultiBiorefinery Project (POCI-01-0145-FEDER-016403) “Multi-purpose strategies for broadband agro-forest and fisheries by-products valorization: a step forward for a truly integrated biorefinery”. Cátia Mendes is grateful for the research grant provided.
Employment or leadership: None declared.
Honorarium: None declared.
References
Alvira, P., Pejó-Tomás, E., Ballesteros, M., Negro, M.J. (2010) Pretreatment technologies for an efficient bioethanol production process based on enzymatic hydrolysis: a review. Bioresour. Technol. 101:4851–4861.10.1016/j.biortech.2009.11.093Search in Google Scholar PubMed
Bozell, J.J., Astner, A.F., Young, T.M., Rials, T.G. (2018) Optimization of components yields and thermal properties by organosolv fractionation of loblolly pine (Pinus taeda) using response surface design. BioEnergy Res. 11:1–13.10.1007/s12155-018-9924-1Search in Google Scholar
Buzala, K.P., Kalinowska, H., Malachowska, E., Przybysz, P. (2017) The utility of selected kraft hardwood and softwood pulps for fuel ethanol. Ind. Crops Prod. 108:824–830.10.1016/j.indcrop.2017.07.038Search in Google Scholar
Cheng, N., Koda, K., Tamai, Y., Yamamoto, Y., Takasuka, T.E., Uraki, Y. (2017) Optimization of simultaneous saccharification and fermentation conditions with amphipathic lignin derivatives for concentrated bioethanol production. Bioresour. Technol. 232:126–132.10.1016/j.biortech.2017.02.018Search in Google Scholar PubMed
Cotana, F., Cavalaglio, G., Gelosia, M., Nicolini, A., Coccia, V., Petrozzi, A. (2014) Production of bioethanol in a second generation prototype from pine wood chips. Energy Procedia 45:42–51.10.1016/j.egypro.2014.01.006Search in Google Scholar
Frankó, B., Galbe, M., Wallber, O. (2016) Bioethanol production from forestry residues: a comparative techno-economic analysis. Appl. Energy 184:727–736.10.1016/j.apenergy.2016.11.011Search in Google Scholar
Kumar, A.K., Sharma, S. (2017) Recent updates on different methods of pretreatment of lignocellulosic feedstocks: a review. Bioresour. Bioprocess. 4:7–26.10.1186/s40643-017-0137-9Search in Google Scholar PubMed PubMed Central
Leskinen, T., Kelley, S.S., Argyropoulos, D.S. (2017) E-beam irradiation & steam explosion as biomass pretreatment, and the complex role of lignin in substrate recalcitrance. Biomass Bioenergy 103:21–28.10.1016/j.biombioe.2017.05.008Search in Google Scholar
Martín-Sampedro, R., Martín, J.A., Eugenio, M.E., Revilla, E., Villar, J.C. (2011) Steam explosion treatment of Eucalyptus globulus wood: influence of operational conditions on chemical and structural modifications. Bioresources 64:4922–4935.Search in Google Scholar
Matsakas, L., Nitsos, C., Raghavendran, V., Yakimenko, O., Persson, G., Olsson, E., Rova, U., Olsoon, L., Christakopoulos, P. (2018) A novel hybrid organosolv: steam explosion method for the efficient fractionation and pretreatment of birch biomass. Biotechnol. Biofuels 11:160–174.10.1186/s13068-018-1163-3Search in Google Scholar PubMed PubMed Central
Rivas, S., Raspolli-Galletti, A.M., Antonetti, C., Santos, V., Parajó, J.C. (2016) Sustainable conversion of Pinus pinaster wood into fuel precursors: a biorefinery approach. Fuel 164:51–58.10.1016/j.fuel.2015.09.085Search in Google Scholar
Seidl, P.R., Goulart, A.K. (2016) Pretreatment processes for lignocellulosic biomass conversion to biofuels and bioproducts. Curr. Opin. Green Sustain. Chem. 2:48–53.10.1016/j.cogsc.2016.09.003Search in Google Scholar
Sluiter, A., Hames, B., Ruiz, R., Scarlata, C., Sluiter, J., Templeton, D., Crocker, D. (2008) Determination of structural carbohydrates and lignin in biomass. National Renewable Energy Laboratory, Golden, CO, 2008. (Technical Report NREL/TP-510-42618).Search in Google Scholar
Stoffel, R.B., Neves, P.V., Felissia, F.E., Ramos, L.P., Gassa, L.M., Area, M.C. (2017) Hemicellulose extraction from slash pine sawdust by steam explosion with sulfuric acid. Biomass Bioenergy 107:93–101.10.1016/j.biombioe.2017.09.019Search in Google Scholar
Valenzuela, R., Priebe, X., Troncoso, E., Ortega, I., Parra, C., Freer, J. (2016) Fiber modifications by organosolv catalyzed with H2SO4 improves SSF of Pinus radiata. Ind. Crops Prod. 86:79–86.10.1016/j.indcrop.2016.03.037Search in Google Scholar
Várnai, A., Huikko, L., Pere, J., Siika-aho, M., Viikari, L. (2011) Synergistic action of xylanase and mannanase improves the total hydrolysis of softwood. Bioresour. Technol. 102:9096–9104.10.1016/j.biortech.2011.06.059Search in Google Scholar PubMed
von Schenck, A., Berglin, N., Uusitalo, J. (2013) Ethanol from Nordic wood raw material by simplified soda cooking pre-treatment. Appl. Energy 102:229–240.10.1016/j.apenergy.2012.10.003Search in Google Scholar
Yamamoto, M., Iakovlev, M., Bankar, S., Tunc, M.S., van Heiningen, A. (2014) Enzymatic hydrolysis of hardwood and softwood harvest residue fibbers released by sulfur dioxide-ethanol-water fractionation. Bioresour. Technol. 167:530–538.10.1016/j.biortech.2014.06.054Search in Google Scholar PubMed
Zabed, H., Sahu, J.N., Suely, A., Boyce, A.N., Faruq, G. (2017) Bioethanol production from renewable sources: current perspectives and technological progress. Renew. Sust. Energy Rev. 71:475–501.10.1016/j.rser.2016.12.076Search in Google Scholar
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