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Sol-gel-derived titanium oxide-cerium oxide biocompatible nanocomposite film for urea sensor

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Abstract

Sol-gel-derived biocompatible titanium oxide–cerium oxide (TiO2-CeO2) nanocomposite film was deposited onto indium tin oxide (ITO)-coated glass substrate by the dip-coating method. This nanobiocomposite film has been characterized using x-ray diffraction, Fourier transform infrared, atomic force microscope, and electrochemical techniques, respectively. The particle size of the TiO2-CeO2 nanobiocomposite film was found to be 23 nm. The urea biosensor fabricated by immobilizing mixed enzyme [urease (Urs) and glutamate dehydrogenase (GLDH)] on this nanobiocomposite showed a response time of 10 s, sensitivity as 0.9165 μAcm-2mM-1, detection limit of 0.166 μM, and negligible effect due to interferants uric acid, cholesterol, glucose, and ascorbic acid. The value of Michaelis-Menten constant (Km) estimated using Lineweaver-Burke plot as 4.8 mM indicated enhancement in the affinity and/or activity of enzyme attached to their nanobiocomposite. This bioelectrode retained 95% of enzyme activity after 6 months at 4 °C.

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Authors and Affiliations

  1. Biomolecular Electronics & Conducting Polymer Research Group, National Physical Laboratory, New Delhi, 110012, India

    Anees A. Ansari, G. Sumana, M. K. Pandey & B. D. Malhotra

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  1. Anees A. Ansari
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  2. G. Sumana
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  3. M. K. Pandey
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  4. B. D. Malhotra
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Correspondence to B. D. Malhotra.

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Ansari, A.A., Sumana, G., Pandey, M.K. et al. Sol-gel-derived titanium oxide-cerium oxide biocompatible nanocomposite film for urea sensor. Journal of Materials Research 24, 1667–1673 (2009). https://doi.org/10.1557/jmr.2009.0212

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  • DOI: https://doi.org/10.1557/jmr.2009.0212

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