Plant Soil Environ., 2012, 58(3):111-120 | DOI: 10.17221/310/2011-PSE

Exogenous nitric oxide alleviates iron-deficiency chlorosis in peanut growing on calcareous soil

X.W. Zhang1, Y.J. Dong1,2, X.K. Qiu1, G.Q. Hu1, Y.H. Wang1, Q.H. Wang1
1 College of Resources and Environment, Shandong Agricultural University, Tai'an, P.R. China
2 Chinese National Engineering Research Center for Slow/Controlled Release Fertilizers,

Sodium nitroprusside (SNP), a nitric oxide (NO) donor, was added into controlled release fertilizer (CRF) or sprayed on leaves to supply NO on iron deficiency stress in peanut (Arachis hypogaea Linn) plants growing on calcareous soils. Iron deficiency reduced plant growth and chlorophyll content. NO improved plant growth and alleviated leaf interveinal chlorosis, and increased the activity of root FeIII reductase and the concentration of available iron in cultured soil, suggesting that NO action could be related to iron availability to the plant. The actual photochemical efficiency (ΦPSII) and photochemical maximum efficiency of PSII (Fv/Fm) were increased, and minimum fluorescence yield (Fo) was decreased under NO-treated condition, which supported the protective effect of NO on photosystem II (PSII) in peanut leaves. NO increased the activities of antioxidant enzymes, and reduced malondialdehyde (MDA) accumulation. These results suggest that exogenous NO could alleviate iron deficiency induced chlorosis of peanut plants growing on calcareous soil.

Keywords: Arachis hypogaea Linn; active iron; FeIII reductase; chlorophyll; antioxidant enzymes

Published: March 31, 2012  Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Zhang XW, Dong YJ, Qiu XK, Hu GQ, Wang YH, Wang QH. Exogenous nitric oxide alleviates iron-deficiency chlorosis in peanut growing on calcareous soil. Plant Soil Environ.. 2012;58(3):111-120. doi: 10.17221/310/2011-PSE.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Ali R., Khan M.N. (1988): Modified butyrometric method for rapid determination of fat in seeds. Journal of the American Oil Chemists' Society, 65: 1951-1952. Go to original source...
    2. Arnon D.I. (1949): Copper enzymes in isolated chloroplasts: polyphenoloxidase in Beta vulgaris. Plant Physiology, 24: 1-15. Go to original source... Go to PubMed...
    3. Beligni M.V., Lamattina L. (2001): Nitric oxide: a non-traditional regulator of plant growth. Trends in Plant Science, 6: 508-509. Go to original source... Go to PubMed...
    4. Beligni M.V., Lamattina L. (2002): Nitric oxide interferes with plant photo-oxidative stress by detoxifying reactive oxygen species. Plant, Cell and Environment, 25: 737-748. Go to original source...
    5. Bradford M.M. (1976): A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72: 248-254. Go to original source...
    6. Fryer M.J., Andrews J.R., Oxborough K., Blowers D.A., Baker N.R. (1998): Relationship between CO2 assimilation, photosynthetic electron transport, and active O2 metabolism in leaves of maize in the field during periods of low temperature. Plant Physiology, 116: 571-580. Go to original source...
    7. Graziano M., Lamattina L. (2007): Nitric oxide accumulation is required for molecular and physiological responses to iron deficiency in tomato roots. Plant Journal, 52: 949-960. Go to original source... Go to PubMed...
    8. Graziano M., Beligni M.V., Lamattina L. (2002): Nitric oxide improves internal iron availability in plants. Plant Physiology, 130: 1852-1859. Go to original source... Go to PubMed...
    9. Liang W.B., Xue S.G., Shen J.H., Wang P. (2010): Effects of manganese stress on photosythesis and chlorophyll fluorescence parameters of Phytolacca americana. Acta Ecologica Sinica, 30: 619-625.
    10. Lindsay W.L., Norvell W.A. (1978): Development of a DTPA soil test for zinc, iron, manganese and copper. Soil Science Society of America Journal, 42: 421-428. Go to original source...
    11. Mengel K. (1994): Iron availability in plant tissues - iron chlorosis on calcareous soils. Plant and Soil, 165: 275-283. Go to original source...
    12. Misra A.N., Misra M., Singh R. (2010): Nitric oxide biochemistry, mode of action and signaling in plants. Journal of Medicinal Plants Research, 4: 2729-2739.
    13. Misra A.N., Misra M., Singh R. (2011): Nitric oxide ameliorates stress responses in plants. Plant, Soil and Environment, 57: 95-100. Go to original source...
    14. Mori S. (1999): Iron acquisition by plants. Current Opinion in Plant Biology, 2: 250-253. Go to original source... Go to PubMed...
    15. Oserkowsky J. (1933): Quantitative relation between chlorophyll and iron in green and chlorotic pear leaves. Plant Physiology, 8: 449-468. Go to original source... Go to PubMed...
    16. Ranieri A., Castagna A., Baldan B., Soldatini G.F. (2001): Iron deficiency differently affects peroxidase isoforms in sunflower. Journal of Experimental Botany, 52: 25-35. Go to original source...
    17. Römheld V., Marschner H. (1983): Mechanism of iron uptake by peanut plants. Plant Physiology, 71: 949-954. Go to original source... Go to PubMed...
    18. Ruan H.H., Shen W.B., Xu L.L. (2004): Nitric oxide modulates the activities of plasma membrane H +-ATPase and PPase in wheat seedling roots and promotes the salt tolerance against salt stress. Acta Botanica Sinica, 46: 415-422.
    19. Sandman G., Malkin R. (1983): Iron-sulfur centers and activities of the photosynthetic electron transport chain in iron-deficient cultures of the blue-green alga Aphanocapsa. Plant Physiology, 73: 724-728. Go to original source... Go to PubMed...
    20. Šrámek F., Dubský M. (2011): Occurrence and correction of lime-induced chlorosis in petunia plants. Plant, Soil and Environment, 57: 180-185. Go to original source...
    21. Sun B.T., Jing Y., Chen K.M., Song L.L., Chen F.J., Zhang L.X. (2007): Protective effect of nitric oxide on iron deficiencyinduced oxidative stress in maize (Zea mays). Journal of Plant Physiology, 164: 536-543. Go to original source... Go to PubMed...
    22. Takker P.N., Kaur N.P. (1984): HCl method for Fe2+ estimation to resolve iron chlorosis in plants. Journal of Plant Nutrition, 7: 81-90. Go to original source...
    23. Vladkova R., Dobrikova A.G., Singh R., Misra A.N., Apostolova E. (2011): Photoelectron transport ability of chloroplast thylakoid membranes treated with NO donor SNP: changes in flash oxygen evolution and chlorophyll fluorescence. Nitric Oxide, 24: 84-90. Go to original source... Go to PubMed...
    24. Xu L.Z., Zhang F.S., Li C.J. (1998): 2,2'-bipyridyl-colorimetric method for measurement of Fe(III) reductase activity in roots of dicotyls. Plant Nutrition and Fertilizer Science, 4: 63-66.
    25. Yang Y., Wang Q.L., Geng M.J., Guo Z.H., Zhao Z. (2011): Rhizosphere pH difference regulated by plasma membrane H+-ATPase is related to differential Al-tolerance of two wheat cultivars. Plant, Soil and Environment, 57: 201-206. Go to original source...
    26. Yi Y., Guerinot M.L. (1996): Genetic evidence that induction of root Fe(III) chelate reductase activity is necessary for iron uptake under iron deficiency. Plant Journal, 10: 835-844. Go to original source... Go to PubMed...
    27. Zhang X.W., Zhang M., Wang Q.H., Qiu X.K., Hu G.Q., Dong Y.J. (2011): Effect of exogenous nitric oxide on physiological characteristic of peanut under iron-deficient stress. Plant Nutrition and Fertilizer Science, 17: 665-673.
    28. Zhang X.Z. (1992): Research Methodology of Crop Physiology. Agriculture Press, Beijing, 208-212.

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content