ISHS Acta Horticulturae 623: XXVI International Horticultural Congress: Plant Genetic Resources, The Fabric of Horticultures Future
POTATO CRYOPRESERVATION IN GERMANY - USING THE DROPLET METHOD FOR THE ESTABLISHMENT OF A NEW LARGE COLLECTION |
| Authors: | E.R.J. Keller, M. Dreiling |
| Keywords: | Solanum tuberosum, culture vessels, in vitro genebanking, regrowth rates |
| DOI: | 10.17660/ActaHortic.2003.623.20 |
| Abstract:
Potato germplasm has been stored in cryopreservation since 1997. The droplet method is applied using 10% dimethyl sulfoxide as cryoprotectant. Explants are fixed at pieces of aluminium foil and frozen ultra-rapidly. Regeneration rates depend on the genotype and range from 20 to 100% per accession. The average survival rate is 72%; the mean regeneration is 48%. The explants are obtained from a collection based on in vitro cycles including storage of microtubers. Donor plantlets are grown from microtubers on MS medium without hormones. Once they have reached lengths of 5 cm, apical explants are dissected for cryopreservation. A comparison has been made with plantlets grown as shoot cultures for several years. Microtuber-derived plantlets gave significantly better results. To further improve the regeneration rates, experiments have been performed using different culture vessel types. | |
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