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A Glass Capillary Microelectrode Based on Capillarity and Its Application to the Detection of l-Glutamate Release from Mouse Brain Slices

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Abstract

A new glass capillary microelectrode for l-glutamate is described using pulled glass capillaries (tip size, ∼12.5 µm) with a very small volume (∼2 µl) of inner solution containing glutamate oxidase (GluOx) and ascorbate oxidase. The operation of the electrode is based on capillary action that samples l-glutamate into the inner solution. The enzyme reaction by GluOx generates hydrogen peroxide that is detected at an Os-gel-HRP polymer modified Pt electrode in a three-electrode configuration. The amperometric response behavior of the electrode was characterized in terms of the capillarity, response time, sensitivity and selectivity for measurements of l-glutamate. The currents at 0 V vs. Ag/AgCl increased linearly with the l-glutamate concentration from 10 to 150 µM for in vitro and in situ calibrations. The response was highly selective to l-glutamate over ascorbate, dopamine, serotonin and other amino acids. The detection of l-glutamate in the extracellular fluids of different regions of mouse hippocampal slices under stimulation of KCl was demonstrated.

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Author notes

  1. Ayumi Hirano

    Present address: Department of Physics and Applied Physics, College of Humanities and Sciences, Nihon University, Sakurajousui, Setagaya, Tokyo, 156-8550, Japan

Authors and Affiliations

  1. Department of Chemistry, College of Humanities and Sciences, Nihon University, Sakurajousui, Setagaya, Tokyo, 156-8550, Japan

    Kumiko Nakajima, Takashi Yamagiwa, Ayumi Hirano & Masao Sugawara

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  1. Kumiko Nakajima
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  2. Takashi Yamagiwa
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  3. Ayumi Hirano
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  4. Masao Sugawara
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Nakajima, K., Yamagiwa, T., Hirano, A. et al. A Glass Capillary Microelectrode Based on Capillarity and Its Application to the Detection of l-Glutamate Release from Mouse Brain Slices. ANAL. SCI. 19, 55–60 (2003). https://doi.org/10.2116/analsci.19.55

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  • DOI: https://doi.org/10.2116/analsci.19.55

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