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Single-Channel Recordings of Gramicidin at Agarose-Supported Bilayer Lipid Membranes Formed by the Tip-Dip and Painting Methods

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Abstract

Agarose-supported BLMs were prepared by the tip-dip and painting methods, and single-channel recordings of gramicidin were examined for the development of an ion-channel sensor. The supported BLMs formed by the tip-dip method had an electric resistance of > 1. 0 × 1011 Ω and a longer lifetime as compared with unsupported ones, which enabled single-channel recordings of gramicidin. The supported BLMs formed by the painting method also enabled single-channel recordings, but the lifetime was shorter than that of unsupported planar BLMs formed by the monolayer folding method.

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Authors and Affiliations

  1. Division of Neurophysiology, National Institute for Medical Research, Mill Hill, London, NW7 1AA, UK

    Ayumi Hirano

  2. Department of Chemistry, College of Humanities and Sciences, Nihon University, Setagaya-ku, Tokyo, 156-8550, Japan

    Yuka Matsuno, Chie Osono, Ayumi Hirano & Masao Sugawara

Authors
  1. Yuka Matsuno
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  2. Chie Osono
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  3. Ayumi Hirano
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  4. Masao Sugawara
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Correspondence to Masao Sugawara.

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Matsuno, Y., Osono, C., Hirano, A. et al. Single-Channel Recordings of Gramicidin at Agarose-Supported Bilayer Lipid Membranes Formed by the Tip-Dip and Painting Methods. ANAL. SCI. 20, 1217–1221 (2004). https://doi.org/10.2116/analsci.20.1217

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  • DOI: https://doi.org/10.2116/analsci.20.1217

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