Abstract
A difference diffusion-based NMR technique and cold-spray ionization mass spectrometry were employed as a solution-based approach for identifying a ligand binding with a protein receptor. The difference diffusion-based NMR technique, called difference NOE-pumping, can directly detect the ligand interacting with a protein receptor. This technique uses a simple pulse sequence and the diffusion filter can easily be optimized. The cold-spray ionization mass spectrometry (CSI-MS), a variant of electrospray ionization mass spectrometry (ESI-MS) operating at low temperature, has been applied to detect the ligand-receptor complex. The efficiency of these techniques for identifying binding ligands is demonstrated with the human serum albumin (HSA)-drug system.
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Seki, H., Sei, Y., Shikii, K. et al. Application of Difference NOE-pumping NMR Technique and Cold-Spray Ionization Mass Spectrometry to Identify a Ligand Binding with a Protein Receptor. ANAL. SCI. 20, 1467–1470 (2004). https://doi.org/10.2116/analsci.20.1467
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DOI: https://doi.org/10.2116/analsci.20.1467