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Glucose Sensor Based on Glucose Oxidase Immobilized by Zirconium Phosphate

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Abstract

Amperometric glucose sensors were fabricated using glucose oxidase (GOx) entrapped in zirconium hydrogenphosphate (ZrP), and their performance was evaluated. Reportedly, α-ZrP is one of the candidates that are expected to improve the stability of enzymes immobilized on solid surfaces.1,2 We intercalated GOxs into ZrP (GOx/ZrP), cast the GOx/ZrP suspension in polyvinylalcohol on a platinum electrode, and dried it in a vacuum oven. The morphological layered structure was investigated by scanning electron microscopy. The enzymatic activities, which were determined by open-circuit potentiometric technique, reached the highest when GOxs were immobilized in ZrP at ca. pH 5. In vitro tests showed good linear responses in the 0 - 25 mM range and the sensitivity of 0.14 nA mM−1 at 0.4 V vs. Ag/AgCl. The sensors, as made, were stable for more than 3 days within a limited deterioration.

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Authors and Affiliations

  1. Department of Chemistry, Sungshin Women’s University, 249-1, Dongsun-dong, Seongbuk-gu, Seoul, 136-742, Korea

    Sejin Park, Taek Dong Chung, Ran-A Jeong & Hankil Boo

  2. Department of Biomedical Engineering, College of Medicine and Institute of Medical and Biological Engineering, Medical Research Center, Seoul National University, 28 Yongon-dong, Chongno-ku, Seoul, 110-744, Korea

    Sejin Park, Sun Kil Kang, Ran-A Jeong, Hankil Boo & Hee Chan Kim

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  1. Sejin Park
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  2. Taek Dong Chung
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  3. Sun Kil Kang
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  4. Ran-A Jeong
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  5. Hankil Boo
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  6. Hee Chan Kim
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Correspondence to Hee Chan Kim.

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Park, S., Chung, T.D., Kang, S.K. et al. Glucose Sensor Based on Glucose Oxidase Immobilized by Zirconium Phosphate. ANAL. SCI. 20, 1635–1638 (2004). https://doi.org/10.2116/analsci.20.1635

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  • DOI: https://doi.org/10.2116/analsci.20.1635

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