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An Anti E. Coli O157:H7 Antibody-Immobilized Microcantilever for the Detection of Escherichia Coli (E. coli)

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Abstract

A silicon microcantilever sensor was developed for the detection of Escherichia coli O157:H7. The microcantilever was modified by anti-E. coli O157:H7 antibodies on the silicon surface of the cantilever. When the aquaria E. coli O157:H7 positive sample is injected into the fluid cell where the microcantilever is held, the microcantilever bends upon the recognition of the E. coli O157:H7 antigen by the antibodies on the surface of the microcantilever. A negative control sample that does not contain E. coli O157:H7 antigen did not cause any bending of the microcantilever. The detection limit of the sensor was 1 × 106 cfu/mL when the assay time was < 2 h.

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Authors and Affiliations

  1. Department of Chemistry and the Institute for Micromanufacturing, Louisiana Tech University, Ruston, Louisiana, 71272, USA

    Jing Zhang & Hai-Feng Ji

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  1. Jing Zhang
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  2. Hai-Feng Ji
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Correspondence to Hai-Feng Ji.

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Zhang, J., Ji, HF. An Anti E. Coli O157:H7 Antibody-Immobilized Microcantilever for the Detection of Escherichia Coli (E. coli). ANAL. SCI. 20, 585–587 (2004). https://doi.org/10.2116/analsci.20.585

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  • DOI: https://doi.org/10.2116/analsci.20.585

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