Nucleic Acids Analysis with Nano-Ag-Tb(III) by a Resonance Light Scattering Technique

Abstract

The nano-Ag-terbium(III)–nucleic acids system was observed by a resonance light scattering (RLS) technique for the first time, and the quantitative analysis of nucleic acids at nanogram levels was established. Studies showed that the RLS intensity of the nano-Ag-terbium(III) system can be obviously enhanced by nucleic acid, which was characterized by the RLS spectrum and the UV-Vis spectrum. In this system, the nanoparticles were only of a definite size and in a limited particle concentration region. Further research indicated that under the optimum conditions, the enhanced intensity of RLS is in proportion to the concentration of nucleic acids in the ranges of 7.0 × 10⁻⁹ g ml⁻¹ to 8.0 × 10⁻⁶ g ml⁻¹ for calf thymus DNA (ctDNA), 2.0 × 10⁻⁸ g ml⁻¹ to 1.0 × 10⁻⁶ g ml⁻¹ for fish sperm DNA (fsDNA) and 1.0 × 10⁻⁹ g ml⁻¹ to 1.0 × 10⁻⁷ g ml⁻¹ for yeast RNA (yRNA). The detection limits were 1.4 ng ml⁻¹ for ctDNA, 1.2 ng ml⁻¹ for fsDNA and 0.85 ng ml⁻¹ for yRNA, respectively. Synthetic and real samples were determined satisfactorily.