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Electrochemical Immunosensor for Carcinoembryonic Antigen Based on Antigen Immobilization in Gold Nanoparticles Modified Chitosan Membrane

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Abstract

A disposable electrochemical immunosensor for carcinoembryonic antigen (CEA) was proposed based on the antigen immobilized in a colloidal gold nanoparticles modified chitosan membrane on the surface of an indium-tin oxide (ITO) electrode. The different membranes were characterized by scanning electron microscope and electrochemical methods. Based on a competitive immunoassay format, the immobilized antigen of the immunosensor was incubated with a horseradish peroxidase (HRP) labeled antibody and sample CEA antigen, and the formed immunoconjugate in the immunosensor was detected by an o-phenylenediamine-H2O2-HRP electrochemical system. Under the optimal experimental conditions, the electrocatalytic current decreased linearly with the competitive mechanism. CEA could be determined in the linear range from 2.0 to 20 ng/ml with a detection limit of 1.0 ng/ml. The prepared CEA immunosensor is not only economic due to the low-cost ITO electrode obtained from industrial mass production, but is also capable with good stability and reproducibility for batch fabrication.

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Authors and Affiliations

  1. College of Chemistry and Molecular Engineering, Qingdao University of Science & Technology, Qingdao, 266042, P. R. China

    Jiehua Lin, Wei Qu & Shusheng Zhang

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  1. Jiehua Lin
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  2. Wei Qu
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  3. Shusheng Zhang
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Correspondence to Shusheng Zhang.

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Lin, J., Qu, W. & Zhang, S. Electrochemical Immunosensor for Carcinoembryonic Antigen Based on Antigen Immobilization in Gold Nanoparticles Modified Chitosan Membrane. ANAL. SCI. 23, 1059–1063 (2007). https://doi.org/10.2116/analsci.23.1059

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  • DOI: https://doi.org/10.2116/analsci.23.1059

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