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Anion- and Cation-Exchange MicroHPLC Utilizing Poly(methacrylates)-coated Monolithic Silica Capillary Columns

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Abstract

A novel method was developed for the preparation of highly efficient anion- and cation-exchange microHPLC columns using an on-column polymerization of methacrylates having amine or sulfonic acid functional groups onto monolithic silica capillary columns modified with 3-methacryloxypropyltriethoxysilane as the anchor groups. The chromatographic evaluation of the columns using nucleic acids, nucleotides, and inorganic anions as samples showed the characteristics of the ion-exchange-type stationary phases. These columns exhibited higher separation efficiency when compared with the conventional particle-packed columns. A capillary column for the simultaneous anion- and cation-exchange separation could be prepared by a step-by-step functionalization. The advantages of this column preparation will include: (1) no need of column packing; (2) no need of the preparation of silane reagents possessing anion- and cation-exchange functionalities; (3) the amount of immobilized polymer could be controlled by changing polymerization conditions. These columns should be suitable for the separation of biologically active compounds by the microHPLC modes.

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References

  1. M. Motokawa, H. Kobayashi, N. Ishizuka, H. Minakuchi, K. Nakanishi, H. Jinnai, K. Hosoya, T. Ikegami, and N. Tanaka, J. Chromatogr., A, 2002, 961, 53.

    Article  CAS  Google Scholar 

  2. H. Kobayashi, T. Ikegami, H. Kimura, T. Hara, D. Tokuda, and N. Tanaka, Anal. Sci., 2006, 22, 491.

    Article  CAS  Google Scholar 

  3. Q.-Z. Luo, Y.-F. Shen, K. K. Hixson, R. Zhao, F. Yang, R. J. Moore, H. M. Mottaz, and R. D. Smith, Anal. Chem., 2005, 77, 5028.

    Article  CAS  Google Scholar 

  4. V. V. Tolstikov, A. Lommen, K. Nakanishi, N. Tanaka, and O. Fiehn, Anal. Chem., 2003, 75, 6737.

    Article  CAS  Google Scholar 

  5. H. Kimura, T. Tanigawa, H. Morisaka, T. Ikegami, K. Hosoya, N. Ishizuka, H. Minakuchi, K. Nakanishi, M. Ueda, K. Cabrera, and N. Tanaka, J. Sep. Sci., 2004, 27, 897.

    Article  CAS  Google Scholar 

  6. T. Ikegami, T. Hara, H. Kimura, H. Kobayashi, K. Hosoya, K. Cabrera, and N. Tanaka, J. Chromatogr., A, 2006, 1106, 112.

    Article  CAS  Google Scholar 

  7. C. J. Venkatramani and Y. Zelechonok, Anal. Chem., 2003, 75, 3484.

    Article  CAS  Google Scholar 

  8. D. Lubda, K. Cabrera, K. Nakanishi, and W. Lindner, Anal. Bioanal. Chem., 2003, 377, 892.

    Article  CAS  Google Scholar 

  9. B. Chankvetadze, C. Yamamoto, M. Kamigaito, N. Tanaka, K. Nakanishi, and Y. Okamoto, J. Chromatogr., A, 2006, 1110, 46.

    Article  CAS  Google Scholar 

  10. T. Ikegami, H. Fujita, K. Horie, K. Hosoya, and N. Tanaka, Anal. Bioanal. Chem., 2006, 386, 578.

    Article  CAS  Google Scholar 

  11. T. Ikegami, K. Horie, J. Jaafar, K. Hosoya, and N. Tanaka, J. Biochem. Biophys. Methods, 2006, in press.

    Google Scholar 

  12. C.-H. Xie, J.-W. Hu, H. Xiao, X.-Y. Su, J. Dong, R.-J. Tian, Z.-K. He, and H.-F. Zou, J. Sep. Sci., 2005, 28, 751.

    Article  CAS  Google Scholar 

  13. T. Ikegami, E. Dicks, H. Kobayashi, H. Morisaka, D. Tokuda, K. Cabrera, K. Hosoya, and N. Tanaka, J. Sep. Sci., 2004, 27, 1292.

    Article  CAS  Google Scholar 

  14. F. Svec, J. Sep. Sci., 2005, 28, 729.

    Article  CAS  Google Scholar 

  15. C. L. Gatlin, R. H. Pieper, S.-T. Huang, E. Mongodin, E. Gebregeorgis, P. P. Parmar, D. J. Clark, H. Alami, L. Papazisi, R. D. Fleischmann, S. R. Gill, and S. N. Peterson, Proteomics, 2006, 6, 1530.

    Article  CAS  Google Scholar 

  16. S. J. Hattan, J. Marchese, N. Khainovski, S. Martin, and P. Juhasz, J. Proteome Res., 2005, 4, 1931.

    Article  CAS  Google Scholar 

  17. K. V. Swanson and J. M. Griffiss, Carbohydr. Res., 2006, 341, 388.

    Article  CAS  Google Scholar 

  18. T. Minamisawa, K. Suzuki, N. Kajimoto, M. Iida, H. Maeda, and J. Hirabayashi, Carbohydr. Res., 2006341, 230.

    Article  CAS  Google Scholar 

  19. B. M. Mayr, O. Kohlbacher, K. Reinert, M. Sturm, C. Groepl, E. Lange, C. Klein, and G. Huber, J. Proteome Res., 2006, 5, 414.

    Article  CAS  Google Scholar 

  20. W. Rozhon, E. Petutschnig, M. Wrzaczek, and C. Jonak, Anal. Bioanal. Chem., 2005, 382, 1620.

    Article  CAS  Google Scholar 

  21. F. N. Bodnaryuk, M. A. Korshunov, and V. S. Mikhlin, Z. Org. Khim., 1972, 8, 1368.

    CAS  Google Scholar 

  22. R. B. van Breemen, C.-R. Huang, Z.-Z. Lu, A. Rimando, H. H. S. Fong, and J. F. Fitzloff, Anal. Chem., 1995, 67, 3985.

    Article  Google Scholar 

  23. M. A. Strege, S. Stevenson, and S. M. Lawrence, Anal. Chem., 2000, 72, 4629.

    Article  CAS  Google Scholar 

  24. A. J. Link, J. Eng, D. M. Schieltz, E. Carmack, G. J. Mize, D. R. Morris, B. M. Garvik, and J. R. III Yates, Nat. Biotechnol., 1999, 17, 676.

    Article  CAS  Google Scholar 

  25. R. Nogueira, M. Laemmerhofer, and W. Lindner, J. Chromatogr., A, 2005, 1089, 158.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biomolecular Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo, Kyoto, 606-8585, Japan

    Tohru Ikegami, Jun Ichimaru, Wataru Kajiwara, Nozomi Nagasawa, Ken Hosoya & Nobuo Tanaka

Authors
  1. Tohru Ikegami
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  2. Jun Ichimaru
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  3. Wataru Kajiwara
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  4. Nozomi Nagasawa
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  5. Ken Hosoya
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  6. Nobuo Tanaka
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Correspondence to Tohru Ikegami.

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Ikegami, T., Ichimaru, J., Kajiwara, W. et al. Anion- and Cation-Exchange MicroHPLC Utilizing Poly(methacrylates)-coated Monolithic Silica Capillary Columns. ANAL. SCI. 23, 109–113 (2007). https://doi.org/10.2116/analsci.23.109

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  • DOI: https://doi.org/10.2116/analsci.23.109

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