Skip to main content
SpringerLink
Log in

Culture and Leukocyte Adhesion Assay of Human Arterial Endothelial Cells in a Glass Microchip

  • Original Papers
  • Published:
Analytical Sciences Aims and scope Submit manuscript

Abstract

Cells are frequently exploited as processing components for integrated chemical systems, such as biochemical reactors and bioassay systems. By culturing vascular endothelial cells (ECs) in integrated chemical devices, vascular models have also been fabricated. Here, we utilized a thermally fused-glass microchip which is chemically and physically stable and favorable for optical detections, and cultured human arterial ECs (HAECs) in it. HAECs reached confluence within 4 days. Survival and tolerance for high shear stress (25 dyn/cm2) of the HAECs were confirmed. Furthermore, HAECs responded to inflammatory cytokine, tumor necrosis facor-α (TNF-α) and attached to more leukocyte cell line, HL-60 cells than unstimulated HAECs. Our developed device can be applied as a human arterial model, and we propose it as a new method for vascular studies.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. G. M. Whitesides, Nature, 2006, 442, 368.

    Article  CAS  PubMed  Google Scholar 

  2. P. S. Dittrich, K. Tachikawa, and A. Manz, Anal. Chem., 2006, 78, 3887.

    Article  CAS  PubMed  Google Scholar 

  3. D. J. Beebe, J. S. Moore, J. M. Bauer, Q. Yu, R. H. Liu, C. Devadoss, and B.-H. Jo, Nature, 2000, 404, 588.

    Article  CAS  PubMed  Google Scholar 

  4. T. Thorsen, S. J. Maerkl, and S. R. Quake, Science, 2002, 298, 580.

    Article  CAS  PubMed  Google Scholar 

  5. A. Groisman, M. Enzelberger, and S. R. Quake, Science, 2003, 300, 955.

    Article  CAS  PubMed  Google Scholar 

  6. J. El-Ali, P. K. Sorger, and K. F. Jensen, Nature, 2006, 442, 403.

    Article  CAS  PubMed  Google Scholar 

  7. D. N. Breslauer, P. J. Lee, and L. P. Lee, Mol. Biosyst., 2006, 2, 97.

    Article  CAS  PubMed  Google Scholar 

  8. S. Takayama, E. Ostuni, P. LeDuc, K. Naruse, D. E. Ingber, and G. M. Whitesides, Nature, 2001, 411, 1016.

    Article  CAS  PubMed  Google Scholar 

  9. E. M. Lucchetta, J. H. Lee, L. A. Fu, N. H. Patel, and R. F. Ismagilov, Nature, 2005, 434, 1134.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. H. Lu, M. A. Schmidt, and K. F. Jensen, Lab Chip, 2005, 5, 23.

    Article  CAS  PubMed  Google Scholar 

  11. W. Gu, X. Zhu, N. Futai, B. S. Cho, and S. Takayama, Proc. Natl. Acad. Sci. U. S. A, 2004, 101, 15861.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. F. K. Balagaddé, L. You, C. L. Hansen, F. H. Arnold, and S. R. Quake, Science, 2005, 309, 137.

    Article  PubMed  Google Scholar 

  13. A. Groisman, C. Lobo, H. Cho, J. K. Campbell, Y. S. Dufour, A. M. Stevens, and A. Levchenko, Nat. Methods, 2005, 2, 685.

    Article  CAS  PubMed  Google Scholar 

  14. K. Morishima, Y. Tanaka, M. Ebara, T. Shimizu, M. Yamato, A. Kikuchi, T. Okano, and T. Kitamori, Sens. Actuators, B, 2006, 119, 345.

    Article  CAS  Google Scholar 

  15. Y. Tanaka, K. Morishima, T. Shimizu, A. Kikuchi, M. Yamato, T. Okano, and T. Kitamori, Lab Chip, 2006, 6, 230.

    Article  CAS  PubMed  Google Scholar 

  16. Y. Tanaka, K. Morishima, T. Shimizu, A. Kikuchi, M. Yamato, T. Okano, and T. Kitamori, Lab Chip, 2006, 6, 362.

    Article  CAS  PubMed  Google Scholar 

  17. Y. Tanaka, K. Sato, T. Shimizu, M. Yamato, T. Okano, and T. Kitamori, Lab Chip, 2007, 7, 207.

    Article  CAS  PubMed  Google Scholar 

  18. T. Nozawa, C. P. Cheng, T. Noda, and W. C. Little, Circulation, 1994, 90, 3047.

    Article  CAS  PubMed  Google Scholar 

  19. H. J. Berger, S. K. Prasad, A. J. Davidoff, D. Pimental, O. Ellingsen, J. D. Marsh, T. W. Smith, and R. A. Kelly. Am. J. Physiol.—Heart Circul. Physiol., 1994, 266, H341.

    Article  CAS  Google Scholar 

  20. Y. Xia, L. M. Buja, R. C. Scarpulla, and J. B. McMillin. Proc. Natl. Acad. Sci. U. S. A., 1997, 94, 11399.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. T. Kitamori, M. Tokeshi, A. Hibara, and K. Sato, Anal. Chem., 2004, 76, 52A.

    Article  CAS  Google Scholar 

  22. K. Sato, A. Hibara, M. Tokeshi, H. Hisamoto, and T. Kitamori, Anal. Sci., 2003, 19, 15.

    Article  CAS  PubMed  Google Scholar 

  23. S. Hiki, K. Mawatari, A. Hibara, M. Tokeshi, and T. Kitamori, Anal. Chem., 2006, 78, 2859.

    Article  CAS  PubMed  Google Scholar 

  24. E. Tamaki, K. Sato, M. Tokeshi, K. Sato, M. Aihara, and T. Kitamori, Anal. Chem., 2002, 74, 1580.

    Article  Google Scholar 

  25. M. Goto, K. Sato, A. Murakami, M. Tokeshi, and T. Kitamori, Anal. Chem., 2005, 77, 2125.

    Article  CAS  PubMed  Google Scholar 

  26. J. W. Song, W. Gu, N. Futai, K. A. Warner, J. E. Nor, and S. Takayama, Anal. Chem., 2005, 77, 3993.

    Article  CAS  PubMed  Google Scholar 

  27. T. D. Oblak, P. Root, and D. M. Spence, Anal. Chem., 2006, 78, 3193.

    Article  CAS  Google Scholar 

  28. P. Mulder, S. Koster, H. J. van der Linden, G. Molema, and E. Verpoorte, in Proceedings of the Micro Total Analysis Systems 2005, ed. K. F. Jensen, J. Han, D. J. Harrison, and J. Voldman, 2005, Vol. 2, Transducers Research Foundation, San Diego, 1383.

  29. M. Shin, K. Matsuda, O. Ishii, H. Terai, M. Kaazempur-Mofrad, J. Borenstein, M. Detmar, and J. P. Vacanti, Biomed. Microdevices, 2004, 6, 269.

    Article  CAS  PubMed  Google Scholar 

  30. A. Hibara, M. Tokeshi, K. Uchiyama, H. Hisamoto, and T. Kitamori, Anal. Sci., 2001, 17, 89.

    Article  CAS  PubMed  Google Scholar 

  31. Y. Tanaka, K. Sato, M. Yamato, T. Okano, and T. Kitamori, J. Chromatogr., A, 2006, 1111, 233.

    Article  CAS  Google Scholar 

  32. M. P. Bevilacqua, J. S. Pober, M. E. Wheeler, R. S. Cotran, and A. Gimbrone Jr., J. Clin. Invest., 1985, 76, 2003.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  33. M. D. Frame and I. H. Sarelius, Microcirculation, 2000, 7, 419.

    Article  CAS  PubMed  Google Scholar 

  34. B. L. Gray, D. K. Lieu, S. D. Collins, R. L. Smith, and A. I. Barakat, Biomed. Microdevices, 2002, 4, 9.

    Article  Google Scholar 

  35. A. Kamiya, R. Bukhari, and T. Togawa, Bull. Math. Biol., 1984, 46, 127.

    Article  CAS  PubMed  Google Scholar 

  36. C. F. Bennett, T. P. Condon, S. Grimm, H. Chan, and M.-Y. Chiang, J. Immunol., 1994, 152, 3530.

    CAS  PubMed  Google Scholar 

  37. K. M. Chrobak, D. R. Potter, and J. Tien, Microvasc. Res., 2006, 71, 185.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Applied Chemistry, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo, 113-8656, Japan

    Yo Tanaka, Yuji Kikukawa & Takehiko Kitamori

  2. Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency, Kawaguchi, Saitama, 332-0012, Japan

    Yo Tanaka & Takehiko Kitamori

  3. Center for NanoBio Integration, The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo, 113-8656, Japan

    Kae Sato & Takehiko Kitamori

  4. Research Institute for Science and Technology, Kogakuin University, 2665-1 Nakanocho, Hachioji, Tokyo, 192-0015, Japan

    Yasuhiko Sugii

  5. Micro Chemistry Group, Optical Science Laboratory, Kanagawa Academy of Science and Technology (KAST), 3-2-1 Sakado, Takatsu, Kawasaki, Kanagawa, 213-0012, Japan

    Takehiko Kitamori

Authors
  1. Yo Tanaka
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yuji Kikukawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kae Sato
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yasuhiko Sugii
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Takehiko Kitamori
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Takehiko Kitamori.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tanaka, Y., Kikukawa, Y., Sato, K. et al. Culture and Leukocyte Adhesion Assay of Human Arterial Endothelial Cells in a Glass Microchip. ANAL. SCI. 23, 261–266 (2007). https://doi.org/10.2116/analsci.23.261

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.2116/analsci.23.261

Search

Navigation