Abstract
In the present work, a sensitive spectroscopic assay based on surface-enhanced Raman spectroscopy (SERS) using gold nanoparticles as substrates was developed for the rapid detection protein–protein interactions. Detection is achieved by specific binding biotin-modification antibodies with protein-stabilized 30 nm gold nanoparticles, followed by the attachment of avidin-modification Raman-active dyes. As a proof-of-principle experiment, a well-known biomolecular recognition system, IgG with protein A, was chosen to establish this new spectroscopic assay. Highly selective recognition of IgG down to 1 ng/ml in solution has been demonstrated.
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Li, T., Guo, L. & Wang, Z. Gold Nanoparticle-based Surface Enhanced Raman Scattering Spectroscopic Assay for the Detection of Protein–Protein Interactions. ANAL. SCI. 24, 907–910 (2008). https://doi.org/10.2116/analsci.24.907
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DOI: https://doi.org/10.2116/analsci.24.907