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Trypsin-Stabilized Fluorescent Gold Nanocluster for Sensitive and Selective Hg2+ Detection

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Abstract

We report on trypsin-stabilized fluorescent gold nanoclusters (Au NCs) for the sensitive and selective detection of Hg2+ ions. The Au NCs have an average size of 1 nm and show a red emission at 645 nm. The photostable properties of the trypsin-stabilized Au NCs were examined, and their photochemical stability was found to be similar to that of CdSe quantum dots. The fluorescence was particularly quenched by Hg2+, and therefore the Au NCs can be used as fluorescent sensors for sensitive and selective Hg2+ detection to a detection limit of 50 ± 10 nM and the quantitative detection of Hg2+ in wide and low concentration range of 50 – 600 nM.

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Authors and Affiliations

  1. Department of Chemistry and Materials Engineering, Faculty of Chemistry, Materials and Bioengineering, Kansai University, 3-3-35 Yamate, 564-8680, Suita, Osaka, Japan

    Hideya Kawasaki, Kouta Yoshimura, Kenji Hamaguchi & Ryuichi Arakawa

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  1. Hideya Kawasaki
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  2. Kouta Yoshimura
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  3. Kenji Hamaguchi
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  4. Ryuichi Arakawa
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Correspondence to Hideya Kawasaki.

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Kawasaki, H., Yoshimura, K., Hamaguchi, K. et al. Trypsin-Stabilized Fluorescent Gold Nanocluster for Sensitive and Selective Hg2+ Detection. ANAL. SCI. 27, 591–596 (2011). https://doi.org/10.2116/analsci.27.591

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  • DOI: https://doi.org/10.2116/analsci.27.591

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